Human Shh-N ELISA Kit

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  • Alternative name

    Sonic hedgehog protein ELISA KIT; HHG-1Cleaved into the following 2 chains:Sonic hedgehog protein N-product ELISA KIT; Sonic hedgehog protein C-productSHH ELISA KIT; SHH ELISA KIT

  • Catalog
    E036172
  • species
    Human
  • GeneShh-N
  • Other Species Mouse Shh-N ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human Shh-N. No significant cross-reactivity or interference between human Shh-N and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human Shh-N is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman Shh-N ELISA Kit allows for the in vitro quantitative determination of Shh-N , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Shh-N has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Shh-N present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Shh-N is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Shh-N bound in the initial step. The color development is stopped and the intensity of the color is measured.



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