Human SLC9A3 ELISA Kit

Alternative name
Human Na(+)/H(+) exchanger 3 ELISA Kit;Human NHE-3 ELISA Kit;Human Solute carrier family 9 member 3 ELISA Kit;Human DIAR8 ELISA Kit;Human NHE3 ELISA Kit;Human solute carrier family 9 member A3 ELISA Kit;Human sodium/hydrogen exchanger 3 ELISA Kit;Human solute carrier family 9 (sodium/hydrogen exchanger) ELISA Kit;Human solute carrier family 9 (sodium/hydrogen exchanger), member 3 ELISA Kit;Human solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 ELISA Kit;
Catalog
E036128
species
Human
GeneSLC9A3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human SLC9A3. No significant cross-reactivity or interference between Human SLC9A3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity2 pg/ml
Intended UseHuman SLC9A3 ELISA Kit allows for the in vitro quantitative determination of SLC9A3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: The Na(+)/H(+) exchanger (NHE) is a ubiquitous protein present in mammalian cells. In higher eukaryotes this integral membrane protein removes one intracellular H(+) for one extracellular Na(+) protecting cells from intracellular acidification. NHE is of essential importance in the myocardium. It prevents intracellular acidosis that inhibits contractility. NHE also plays a key role in damage to the mammalian myocardium that occurs during ischemia and reperfusion and is involved in hypertrophy of the myocardium. NHE is composed of a membrane bound domain of approximately 500 amino acids plus a hydrophilic regulatory cytoplasmic domain of approximately 315 amino acids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NHE3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NHE3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NHE3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NHE3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Sodium/hydrogen exchanger 3 Protein information
Uniprot ID SL9A3_HUMAN
Uniprot AC P48764; B7ZKR2; E9PF67; Q3MIW3;
UniGene Hs.658120;
GeneID 6550
KEGG hsa:6550;
Human Sodium/hydrogen exchanger 3 Protein SEQUENCE
SEQUENCE 834 AA; 92855 MW; 3E7CE3267F6E86F7 CRC64;
MWGLGARGPD RGLLLALALG GLARAGGVEV EPGGAHGESG GFQVVTFEWA
HVQDPYVIAL WILVASLAKI GFHLSHKVTS VVPESALLIV LGLVLGGIVW
AADHIASFTL TPTVFFFYLL PPIVLDAGYF MPNRLFFGNL GTILLYAVVG
TVWNAATTGL SLYGVFLSGL MGDLQIGLLD FLLFGSLMAA VDPVAVLAVF
EEVHVNEVLF IIVFGESLLN DAVTVVLYNV FESFVALGGD NVTGVDCVKG
IVSFFVVSLG GTLVGVVFAF LLSLVTRFTK HVRIIEPGFV FIISYLSYLT
SEMLSLSAIL AITFCGICCQ KYVKANISEQ SATTVRYTMK MLASSAETII
FMFLGISAVN PFIWTWNTAF VLLTLVFISV YRAIGVVLQT WLLNRYRMVQ
LEPIDQVVLS YGGLRGAVAF ALVVLLDGDK VKEKNLFVST TIIVVFFTVI
FQGLTIKPLV QWLKVKRSEH REPRLNEKLH GRAFDHILSA IEDISGQIGH
NYLRDKWSHF DRKFLSRVLM RRSAQKSRDR ILNVFHELNL KDAISYVAEG
ERRGSLAFIR SPSTDNVVNV DFTPRSSTVE ASVSYLLREN VSAVCLDMQS
LEQRRRSIRD AEDMVTHHTL QQYLYKPRQE YKHLYSRHEL TPTEDEKQDR
EIFHRTMRKR LESFKSTKLG LNQNKKAAKL YKRERAQKRR NSSIPNGKLP
MESPAQNFTI KEKDLELSDT EEPPNYDEEM SGGIEFLASV TKDTASDSPA
GIDNPVFSPD EALDRSLLAR LPPWLSPGET VVPSQRARTQ IPYSPGTFCR
LMPFRLSSKS VDSFLQADGP EERPPAALPE STHM
UCSC uc003jbe.3; human. [P48764-1];

Category

Human SLC9A3 ELISA Kit