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Human ARG2 ELISA Kit

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  • Alternative name

    Human kidney-type arginase ELISA Kit;Human non-hepatic arginase ELISA Kit;Human type II arginase ELISA Kit;Human arginase 2 ELISA Kit;Human arginase-2, mitochondrial ELISA Kit;Human L-arginine amidinohydrolase ELISA Kit;Human L-arginine ureahydrolase ELISA Kit;Human arginase, type II ELISA Kit;Human kidney arginase ELISA Kit;Human nonhepatic arginase ELISA Kit;
  • Catalog
    E003600
  • species
    Human
  • GeneARG2
  • Standard CurveHuman ARG2 ELISA Kit
  • Other Species Mouse Arg2 ELISA KitBovine ARG2 ELISA KitRabbit ARG2 ELISA KitRat Arg2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ARG2. No significant cross-reactivity or interference between ARG2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARG2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman ARG2 ELISA Kit allows for the in vitro quantitative determination of ARG2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the Assay: ARG2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARG2 antibody and an ARG2-HRP conjugate. The assay sample and buffer are incubated together with ARG2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARG2 concentration since ARG2 from samples and ARG2-HRP conjugate compete for the anti-ARG2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ARG2 from the sample, fewer sites are left to bind ARG2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARG2 concentration in each sample is interpolated from this standard curve.
  • Human Arginase-2, mitochondrial Protein information
  • Uniprot ID ARGI2_HUMAN
  • Uniprot AC P78540; B2R690; Q6FHY8;
  • UniGene Hs.226007;
  • GeneID 384
  • KEGG hsa:384;
  • Human Arginase-2, mitochondrial Protein SEQUENCE

  • SEQUENCE 354 AA; 38578 MW; 1624FAC7D515C68B CRC64;

    MSLRGSLSRL LQTRVHSILK KSVHSVAVIG APFSQGQKRK GVEHGPAAIR

    EAGLMKRLSS LGCHLKDFGD LSFTPVPKDD LYNNLIVNPR SVGLANQELA

    EVVSRAVSDG YSCVTLGGDH SLAIGTISGH ARHCPDLCVV WVDAHADINT

    PLTTSSGNLH GQPVSFLLRE LQDKVPQLPG FSWIKPCISS ASIVYIGLRD

    VDPPEHFILK NYDIQYFSMR DIDRLGIQKV MERTFDLLIG KRQRPIHLSF

    DIDAFDPTLA PATGTPVVGG LTYREGMYIA EEIHNTGLLS ALDLVEVNPQ

    LATSEEEAKT TANLAVDVIA SSFGQTREGG HIVYDQLPTP SSPDESENQA

    RVRI

  • UCSC uc001xjs.4; human.;


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