Human ACR ELISA Kit

Alternative name
Human ACRS ELISA Kit;Human acrosin ELISA Kit;Human acrosin light and heavy chain prepropeptide ELISA Kit;Human preproacrosin ELISA Kit;Human proacrosin ELISA Kit;
Catalog
E000359
species
Human
GeneACR
Standard Curve
Other Species Human ACR-Ab ELISA Kit Mouse Acr ELISA Kit Goat ACR ELISA Kit Turkey ACR ELISA Kit Porcine ACR ELISA Kit Rabbit ACR ELISA Kit Rat Acr ELISA Kit Sheep ACR ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ACR. No significant cross-reactivity or interference between ACR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACR and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ACR ELISA Kit allows for the in vitro quantitative determination of ACR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses: This ACR ELIACR kit is a 1.5 hour solid-phase ELIACR designed for the quantitative determination of Human ACR. This ELIACR kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ACR ELIACR kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ACR antibody and an ACR-HRP conjugate. The assay sample and buffer are incubated together with ACR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACR concentration since ACR from samples and ACR-HRP conjugate compete for the anti-ACR antibody binding site. Since the number of sites is limited, as more sites are occupied by ACR from the sample, fewer sites are left to bind ACR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACR concentration in each sample is interpolated from this standard curve.
Human Acrosin heavy chain Protein information
Uniprot ID ACRO_HUMAN
Uniprot AC P10323; Q6ICK2;
UniGene Hs.370870;
GeneID 49
KEGG hsa:49;
Human Acrosin heavy chain Protein SEQUENCE
SEQUENCE 421 AA; 45847 MW; 7A2F7ECEC98008FA CRC64;
MVEMLPTAIL LVLAVSVVAK DNATCDGPCG LRFRQNPQGG VRIVGGKAAQ
HGAWPWMVSL QIFTYNSHRY HTCGGSLLNS RWVLTAAHCF VGKNNVHDWR
LVFGAKEITY GNNKPVKAPL QERYVEKIII HEKYNSATEG NDIALVEITP
PISCGRFIGP GCLPHFKAGL PRGSQSCWVA GWGYIEEKAP RPSSILMEAR
VDLIDLDLCN STQWYNGRVQ PTNVCAGYPV GKIDTCQGDS GGPLMCKDSK
ESAYVVVGIT SWGVGCARAK RPGIYTATWP YLNWIASKIG SNALRMIQSA
TPPPPTTRPP PIRPPFSHPI SAHLPWYFQP PPRPLPPRPP AAQPRPPPSP
PPPPPPPASP LPPPPPPPPP TPSSTTKLPQ GLSFAKRLQQ LIEVLKGKTY
SDGKNHYDME TTELPELTST S
UCSC uc003bnh.5; human.;

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Human ACR-Ab ELISA Kit

Category

Human ACR ELISA Kit

Human ACRS ELISA Kit;Human acrosin ELISA Kit;Human acrosin light and heavy chain prepropeptide ELISA Kit;Human preproacrosin ELISA Kit;Human proacrosin ELISA Kit;