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Human SLC12A1 ELISA Kit

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  • Alternative name

    Human bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 ELISA Kit;Human kidney-specific Na-K-Cl symporter ELISA Kit;Human NKCC2 ELISA Kit;Human BSC1 ELISA Kit;Human solute carrier family 12 member 1 ELISA Kit;Human NKCC2A variant A ELISA Kit;Human Na-K-2Cl cotransporter ELISA Kit;Human solute carrier family 12 (sodium/potassium/chloride transporter), member 1 ELISA Kit;Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 ELISA Kit;
  • Catalog
    E035844
  • species
    Human
  • GeneSLC12A1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.188ng/ml
  • Intended UseHuman SLC12A1 ELISA Kit allows for the in vitro quantitative determination of SLC12A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human SLC12A1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SLC12A1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SLC12A1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman SLC12A1 can be calculated.
  • Human Solute carrier family 12 member 1 Protein information
  • Uniprot ID S12A1_HUMAN
  • Uniprot AC Q13621; A8JYA2; E9PDW4;
  • UniGene Hs.123116; Hs.605373;
  • GeneID 6557
  • KEGG hsa:6557;
  • Human Solute carrier family 12 member 1 Protein SEQUENCE

  • SEQUENCE 1099 AA; 121450 MW; C1C868B7F8B563AC CRC64;

    MSLNNSSNVF LDSVPSNTNR FQVSVINENH ESSAAADDNT DPPHYEETSF

    GDEAQKRLRI SFRPGNQECY DNFLQSGETA KTDASFHAYD SHTNTYYLQT

    FGHNTMDAVP KIEYYRNTGS ISGPKVNRPS LLEIHEQLAK NVAVTPSSAD

    RVANGDGIPG DEQAENKEDD QAGVVKFGWV KGVLVRCMLN IWGVMLFIRL

    SWIVGEAGIG LGVLIILLST MVTSITGLST SAIATNGFVR GGGAYYLISR

    SLGPEFGGSI GLIFAFANAV AVAMYVVGFA ETVVDLLKES DSMMVDPTND

    IRIIGSITVV ILLGISVAGM EWEAKAQVIL LVILLIAIAN FFIGTVIPSN

    NEKKSRGFFN YQASIFAENF GPRFTKGEGF FSVFAIFFPA ATGILAGANI

    SGDLEDPQDA IPRGTMLAIF ITTVAYLGVA ICVGACVVRD ATGNMNDTII

    SGMNCNGSAA CGLGYDFSRC RHEPCQYGLM NNFQVMSMVS GFGPLITAGI

    FSATLSSALA SLVSAPKVFQ ALCKDNIYKA LQFFAKGYGK NNEPLRGYIL

    TFLIAMAFIL IAELNTIAPI ISNFFLASYA LINFSCFHAS YAKSPGWRPA

    YGIYNMWVSL FGAVLCCAVM FVINWWAAVI TYVIEFFLYV YVTCKKPDVN

    WGSSTQALSY VSALDNALEL TTVEDHVKNF RPQCIVLTGG PMTRPALLDI

    THAFTKNSGL CICCEVFVGP RKLCVKEMNS GMAKKQAWLI KNKIKAFYAA

    VAADCFRDGV RSLLQASGLG RMKPNTLVIG YKKNWRKAPL TEIENYVGII

    HDAFDFEIGV VIVRISQGFD ISQVLQVQEE LERLEQERLA LEATIKDNEC

    EEESGGIRGL FKKAGKLNIT KTTPKKDGSI NTSQSMHVGE FNQKLVEAST

    QFKKKQEKGT IDVWWLFDDG GLTLLIPYIL TLRKKWKDCK LRIYVGGKIN

    RIEEEKIVMA SLLSKFRIKF ADIHIIGDIN IRPNKESWKV FEEMIEPYRL

    HESCKDLTTA EKLKRETPWK ITDAELEAVK EKSYRQVRLN ELLQEHSRAA

    NLIVLSLPVA RKGSISDLLY MAWLEILTKN LPPVLLVRGN HKNVLTFYS

  • UCSC uc001zwn.5; human. [Q13621-1];



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