SpecificityThis assay has high sensitivity and excellent specificity for detection of S100B. No significant cross-reactivity or interference between S100B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between S100B and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman S-100B ELISA Kit allows for the in vitro quantitative determination of S-100B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyHuman ELISA Kit
Product Description specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This S100B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human S100B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY S100B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S100B antibody and an S100B-HRP conjugate. The assay sample and buffer are incubated together with S100B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100B concentration since S100B from samples and S100B-HRP conjugate compete for the anti-S100B antibody binding site. Since the number of sites is limited, as more sites are occupied by S100B from the sample, fewer sites are left to bind S100B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100B concentration in each sample is interpolated from this standard curve.