SpecificityThis assay has high sensitivity and excellent specificity for detection of Soluble P53. No significant cross-reactivity or interference between Soluble P53 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Soluble P53 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman sP53 ELISA Kit allows for the in vitro quantitative determination of sP53 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: Soluble P53 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Soluble P53 antibody and an Soluble P53-HRP conjugate. The assay sample and buffer are incubated together with Soluble P53-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Soluble P53 concentration since Soluble P53 from samples and Soluble P53-HRP conjugate compete for the anti-Soluble P53 antibody binding site. Since the number of sites is limited, as more sites are occupied by Soluble P53 from the sample, fewer sites are left to bind Soluble P53-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Soluble P53 concentration in each sample is interpolated from this standard curve.