-
Alternative name
Multidrug resistance-associated protein 5 ELISA KIT; ATP-binding cassette sub-family C member 5 ELISA KIT; Multi-specific organic anion transporter C ELISA KIT; MOAT-C ELISA KIT; SMRP ELISA KIT; pABC11ABCC5 ELISA KIT; MRP5 ELISA KIT; MOAT-C ELISA KIT
-
Catalog
E035753
-
species
Human
-
GeneSMRP
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity<0.188pmol/ml
-
Intended UseHuman SMRP ELISA Kit allows for the in vitro quantitative determination of SMRP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human SMRP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SMRP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SMRP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human SMRP can be calculated.
-