Human Sil 6R/cd126 ELISA Kit

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  • Alternative name

    Interleukin-6 receptor subunit alpha ELISA KIT; IL-6R 1 ELISA KIT; Membrane glycoprotein 80 ELISA KIT; gp80 ELISA KIT; CD_antigen: CD126IL6R ELISA KIT; IL-6 receptor subunit alpha ELISA KIT; IL-6R subunit alpha ELISA KIT; IL-6R-alpha ELISA KIT; IL-6RA ELISA KIT; gp80 ELISA KIT

  • Catalog
  • species
  • GeneSil 6R/cd126
  • Standard CurveHuman Sil 6R/cd126 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman Sil 6R/cd126 ELISA Kit allows for the in vitro quantitative determination of Sil 6R/cd126 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    Principle of the Assay: SIL-6R/CD126 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SIL-6R/CD126. Standards or samples are then added to the microtiter plate wells and SIL-6R/CD126 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SIL-6R/CD126 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SIL-6R/CD126 are added to each well to "sandwich" the SIL-6R/CD126 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SIL-6R/CD126 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SIL-6R/CD126 concentration in each sample is interpolated from this standard curve.

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