Intended UseHuman sCD146 ELISA Kit allows for the in vitro quantitative determination of sCD146 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntroduction: Soluble CD146, a novel endothelial marker, is increased in physiopathological settings linked to endothelial junctional alteration. sCD146 circulates in the plasma of healthy subjects. Modifications of its basal levels could reflect alterations of junctional functions such as vascular permeability. sCD146 involved in monocyte transendothelial migration during inflammation. TNF can increased the release of a soluble form (sCD146) through a metalloproteinase-dependent mechanism.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to sCD146. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for sCD146 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain sCD146, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of sCD146 in the samples is then determined by comparing the O.D. of the samples to the standard curve.