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Human ALOX15 ELISA Kit

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  • Alternative name

    Human 15-LOX ELISA Kit;Human 15-LOX-1 ELISA Kit;Human 12/15-lipoxygenase ELISA Kit;Human arachidonate 12-lipoxygenase, leukocyte-type ELISA Kit;Human 12-LOX ELISA Kit;Human arachidonate omega-6 lipoxygenase ELISA Kit;Human LOG15 ELISA Kit;Human 15LOX-1 ELISA Kit;Human arachidonate 15-lipoxygenase ELISA Kit;Human 15-lipooxygenase-1 ELISA Kit;
  • Catalog
    E003539
  • species
    Human
  • GeneALOX15
  • Standard CurveHuman ALOX15 ELISA Kit
  • Other Species Mouse Alox15 ELISA KitBovine ALOX15 ELISA KitPorcine ALOX15 ELISA KitRabbit ALOX15 ELISA KitRat Alox15 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Arachidonate-15-Lipoxygenase (ALOX15). No significant cross-reactivity or interference between Arachidonate-15-Lipoxygenase (ALOX15) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.55ng/mL.
  • Intended UseHuman ALOX15 ELISA Kit allows for the in vitro quantitative determination of ALOX15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-15-Lipoxygenase (ALOX15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-15-Lipoxygenase (ALOX15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-15-Lipoxygenase (ALOX15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-15-Lipoxygenase (ALOX15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Arachidonate 15-lipoxygenase Protein information
  • Uniprot ID LOX15_HUMAN
  • Uniprot AC P16050; A8K2P4; B7ZA11; Q8N6R7; Q99657;
  • UniGene Hs.73809;
  • GeneID 246
  • KEGG hsa:246;
  • Human Arachidonate 15-lipoxygenase Protein SEQUENCE

  • SEQUENCE 662 AA; 74804 MW; 9ACF7FE7863A045C CRC64;

    MGLYRIRVST GASLYAGSNN QVQLWLVGQH GEAALGKRLW PARGKETELK

    VEVPEYLGPL LFVKLRKRHL LKDDAWFCNW ISVQGPGAGD EVRFPCYRWV

    EGNGVLSLPE GTGRTVGEDP QGLFQKHREE ELEERRKLYR WGNWKDGLIL

    NMAGAKLYDL PVDERFLEDK RVDFEVSLAK GLADLAIKDS LNVLTCWKDL

    DDFNRIFWCG QSKLAERVRD SWKEDALFGY QFLNGANPVV LRRSAHLPAR

    LVFPPGMEEL QAQLEKELEG GTLFEADFSL LDGIKANVIL CSQQHLAAPL

    VMLKLQPDGK LLPMVIQLQL PRTGSPPPPL FLPTDPPMAW LLAKCWVRSS

    DFQLHELQSH LLRGHLMAEV IVVATMRCLP SIHPIFKLII PHLRYTLEIN

    VRARTGLVSD MGIFDQIMST GGGGHVQLLK QAGAFLTYSS FCPPDDLADR

    GLLGVKSSFY AQDALRLWEI IYRYVEGIVS LHYKTDVAVK DDPELQTWCR

    EITEIGLQGA QDRGFPVSLQ ARDQVCHFVT MCIFTCTGQH ASVHLGQLDW

    YSWVPNAPCT MRLPPPTTKD ATLETVMATL PNFHQASLQM SITWQLGRRQ

    PVMVAVGQHE EEYFSGPEPK AVLKKFREEL AALDKEIEIR NAKLDMPYEY

    LRPSVVENSV AI

  • UCSC uc002fyh.4; human. [P16050-1];



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