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Human ALOX12 ELISA Kit

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  • Alternative name

    Human 12S-LOX ELISA Kit;Human 12S-lipoxygenase ELISA Kit;Human lipoxin synthase 12-LO ELISA Kit;Human platelet-type lipoxygenase 12 ELISA Kit;Human 12LO ELISA Kit;Human LOG12 ELISA Kit;Human 12-LOX ELISA Kit;Human arachidonate 12-lipoxygenase, 12S type ELISA Kit;Human arachidonate 12-lipoxygenase, 12S-type ELISA Kit;Human 12(S)-lipoxygenase ELISA Kit;Human platelet 12-LOX ELISA Kit;Human platelet-type 12-lipoxygenase ELISA Kit;
  • Catalog
    E003538
  • species
    Human
  • GeneALOX12
  • Standard CurveHuman ALOX12 ELISA Kit
  • Other Species Mouse Alox12 ELISA KitRat Alox12 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Arachidonate-12-Lipoxygenase (ALOX12). No significant cross-reactivity or interference between Arachidonate-12-Lipoxygenase (ALOX12) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.116ng/mL.
  • Intended UseHuman ALOX12 ELISA Kit allows for the in vitro quantitative determination of ALOX12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-12-Lipoxygenase (ALOX12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-12-Lipoxygenase (ALOX12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Arachidonate 12-lipoxygenase, 12S-type Protein information
  • Uniprot ID LOX12_HUMAN
  • Uniprot AC P18054; O95569; Q6ISF8; Q9UQM4;
  • UniGene Hs.654431;
  • GeneID 239
  • KEGG hsa:239;
  • Human Arachidonate 12-lipoxygenase, 12S-type Protein SEQUENCE

  • SEQUENCE 663 AA; 75694 MW; C4D6D5B320666A77 CRC64;

    MGRYRIRVAT GAWLFSGSYN RVQLWLVGTR GEAELELQLR PARGEEEEFD

    HDVAEDLGLL QFVRLRKHHW LVDDAWFCDR ITVQGPGACA EVAFPCYRWV

    QGEDILSLPE GTARLPGDNA LDMFQKHREK ELKDRQQIYC WATWKEGLPL

    TIAADRKDDL PPNMRFHEEK RLDFEWTLKA GALEMALKRV YTLLSSWNCL

    EDFDQIFWGQ KSALAEKVRQ CWQDDELFSY QFLNGANPML LRRSTSLPSR

    LVLPSGMEEL QAQLEKELQN GSLFEADFIL LDGIPANVIR GEKQYLAAPL

    VMLKMEPNGK LQPMVIQIQP PNPSSPTPTL FLPSDPPLAW LLAKSWVRNS

    DFQLHEIQYH LLNTHLVAEV IAVATMRCLP GLHPIFKFLI PHIRYTMEIN

    TRARTQLISD GGIFDKAVST GGGGHVQLLR RAAAQLTYCS LCPPDDLADR

    GLLGLPGALY AHDALRLWEI IARYVEGIVH LFYQRDDIVK GDPELQAWCR

    EITEVGLCQA QDRGFPVSFQ SQSQLCHFLT MCVFTCTAQH AAINQGQLDW

    YAWVPNAPCT MRMPPPTTKE DVTMATVMGS LPDVRQACLQ MAISWHLSRR

    QPDMVPLGHH KEKYFSGPKP KAVLNQFRTD LEKLEKEITA RNEQLDWPYE

    YLKPSCIENS VTI

  • UCSC uc002gdx.4; human.;



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