Human ALOXE3 ELISA Kit

Alternative name
Human epidermis-type lipoxygenase 3 ELISA Kit;Human epidermal LOX-3 ELISA Kit;Human e-LOX-3 ELISA Kit;Human eLOX-3 ELISA Kit;Human hydroperoxy icosatetraenoate dehydratase ELISA Kit;Human ARCI3 ELISA Kit;Human E-LOX ELISA Kit;Human eLOX3 ELISA Kit;Human arachidonate lipoxygenase 3 ELISA Kit;Human hydroperoxide isomerase ALOXE3 ELISA Kit;Human epidermal lipoxygenase ELISA Kit;
Catalog
E003536
species
Human
GeneALOXE3
Standard Curve
Other Species Mouse Aloxe3 ELISA Kit Rat Aloxe3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Arachidonate Lipoxygenase 3 (ALOXE3). No significant cross-reactivity or interference between Arachidonate Lipoxygenase 3 (ALOXE3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.114ng/mL.
Intended UseHuman ALOXE3 ELISA Kit allows for the in vitro quantitative determination of ALOXE3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate Lipoxygenase 3 (ALOXE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate Lipoxygenase 3 (ALOXE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate Lipoxygenase 3 (ALOXE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate Lipoxygenase 3 (ALOXE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Hydroperoxide isomerase ALOXE3 Protein information
Uniprot ID LOXE3_HUMAN
Uniprot AC Q9BYJ1; B2R981; B7Z3W0; Q3ZB74; Q9H4F2; Q9HC22;
UniGene Hs.232770;
GeneID 59344
KEGG hsa:59344;
Human Hydroperoxide isomerase ALOXE3 Protein SEQUENCE
SEQUENCE 711 AA; 80543 MW; BDED1E4ED5CF6783 CRC64;
MAVYRLCVTT GPYLRAGTLD NISVTLVGTC GESPKQRLDR MGRDFAPGSV
QKYKVRCTAE LGELLLLRVH KERYAFFRKD SWYCSRICVT EPDGSVSHFP
CYQWIEGYCT VELRPGTART ICQDSLPLLL DHRTRELRAR QECYRWKIYA
PGFPCMVDVN SFQEMESDKK FALTKTTTCV DQGDSSGNRY LPGFPMKIDI
PSLMYMEPNV RYSATKTISL LFNAIPASLG MKLRGLLDRK GSWKKLDDMQ
NIFWCHKTFT TKYVTEHWCE DHFFGYQYLN GVNPVMLHCI SSLPSKLPVT
NDMVAPLLGQ DTCLQTELER GNIFLADYWI LAEAPTHCLN GRQQYVAAPL
CLLWLSPQGA LVPLAIQLSQ TPGPDSPIFL PTDSEWDWLL AKTWVRNSEF
LVHENNTHFL CTHLLCEAFA MATLRQLPLC HPIYKLLLPH TRYTLQVNTI
ARATLLNPEG LVDQVTSIGR QGLIYLMSTG LAHFTYTNFC LPDSLRARGV
LAIPNYHYRD DGLKIWAAIE SFVSEIVGYY YPSDASVQQD SELQAWTGEI
FAQAFLGRES SGFPSRLCTP GEMVKFLTAI IFNCSAQHAA VNSGQHDFGA
WMPNAPSSMR QPPPQTKGTT TLKTYLDTLP EVNISCNNLL LFWLVSQEPK
DQRPLGTYPD EHFTEEAPRR SIAAFQSRLA QISRDIQERN QGLALPYTYL
DPPLIENSVS I
UCSC uc010cnr.4; human. [Q9BYJ1-1];

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Human ALOXE3 ELISA Kit