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Human SIRT5 ELISA Kit

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  • Alternative name

    Human Regulatory protein SIR2 homolog 5 ELISA Kit;Human SIR2-like protein 5 ELISA Kit;Human SIR2L5 ELISA Kit;Human sirtuin 5 ELISA Kit;Human NAD-dependent protein deacylase sirtuin-5, mitochondrial ELISA Kit;Human NAD-dependent deacetylase sirtuin-5 ELISA Kit;Human NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial ELISA Kit;Human silent mating type information regulation 2, S.cerevisiae, homolog 5 ELISA Kit;Human sir2-like 5 ELISA Kit;Human sirtuin type 5 ELISA Kit;
  • Catalog
    E035348
  • species
    Human
  • GeneSIRT5
  • Other Species Mouse Sirt5 ELISA KitBovine SIRT5 ELISA KitCanine SIRT5 ELISA KitChicken SIRT5 ELISA KitRat Sirt5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human SIRT5. No significant cross-reactivity or interference between human SIRT5 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human SIRT5 is typically less than 0.78pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman SIRT5 ELISA Kit allows for the in vitro quantitative determination of SIRT5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SIRT5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIRT5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human NAD-dependent protein deacylase sirtuin-5, mitochondrial Protein information
  • Uniprot ID SIR5_HUMAN
  • Uniprot AC Q9NXA8; B4DFM4; B4DYJ5; F5H5Z9; Q5T294; Q5T295; Q9Y6E6;
  • UniGene Hs.567431; Hs.594133;
  • GeneID 23408
  • KEGG hsa:23408;
  • Human NAD-dependent protein deacylase sirtuin-5, mitochondrial Protein SEQUENCE

  • SEQUENCE 310 AA; 33881 MW; 022DA32CDB43AC3A CRC64;

    MRPLQIVPSR LISQLYCGLK PPASTRNQIC LKMARPSSSM ADFRKFFAKA

    KHIVIISGAG VSAESGVPTF RGAGGYWRKW QAQDLATPLA FAHNPSRVWE

    FYHYRREVMG SKEPNAGHRA IAECETRLGK QGRRVVVITQ NIDELHRKAG

    TKNLLEIHGS LFKTRCTSCG VVAENYKSPI CPALSGKGAP EPGTQDASIP

    VEKLPRCEEA GCGGLLRPHV VWFGENLDPA ILEEVDRELA HCDLCLVVGT

    SSVVYPAAMF APQVAARGVP VAEFNTETTP ATNRFRFHFQ GPCGTTLPEA

    LACHENETVS

  • UCSC uc003naw.4; human. [Q9NXA8-1];


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