Human SIRT1 ELISA Kit

Alternative name
Human hSIRT1 ELISA Kit;Human regulatory protein SIR2 homolog 1 ELISA Kit;Human SIR2-like protein 1 ELISA Kit;Human hSIR2 ELISA Kit;Human 75SirT1 ELISA Kit;Human SIR2L1 ELISA Kit;Human SIR2 ELISA Kit;Human SIR2alpha ELISA Kit;Human sirtuin 1 ELISA Kit;Human NAD-dependent protein deacetylase sirtuin-1 ELISA Kit;Human sirtuin type 1 ELISA Kit;
Catalog
E035342
species
Human
GeneSIRT1
SpecificityThis kit recognizes natural and recombinant Human SIRT1. No significant cross-reactivity or interference between Human SIRT1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of Human SIRT1 is 0.188ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
Intended UseHuman SIRT1 ELISA Kit allows for the in vitro quantitative determination of SIRT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SIRT1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SIRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SIRT1. You can calculate the concentration of SIRT1 in the samples by comparing the OD of the samples to the standard curve.
Human SirtT1 75 kDa fragment Protein information
Uniprot ID SIR1_HUMAN
Uniprot AC Q96EB6; Q2XNF6; Q5JVQ0; Q9GZR9; Q9Y6F0;
UniGene Hs.369779;
GeneID 23411
KEGG hsa:23411;
Human SirtT1 75 kDa fragment Protein SEQUENCE
SEQUENCE 747 AA; 81681 MW; 2D3BEA6D73DA229F CRC64;
MADEAALALQ PGGSPSAAGA DREAASSPAG EPLRKRPRRD GPGLERSPGE
PGGAAPEREV PAAARGCPGA AAAALWREAE AEAAAAGGEQ EAQATAAAGE
GDNGPGLQGP SREPPLADNL YDEDDDDEGE EEEEAAAAAI GYRDNLLFGD
EIITNGFHSC ESDEEDRASH ASSSDWTPRP RIGPYTFVQQ HLMIGTDPRT
ILKDLLPETI PPPELDDMTL WQIVINILSE PPKRKKRKDI NTIEDAVKLL
QECKKIIVLT GAGVSVSCGI PDFRSRDGIY ARLAVDFPDL PDPQAMFDIE
YFRKDPRPFF KFAKEIYPGQ FQPSLCHKFI ALSDKEGKLL RNYTQNIDTL
EQVAGIQRII QCHGSFATAS CLICKYKVDC EAVRGDIFNQ VVPRCPRCPA
DEPLAIMKPE IVFFGENLPE QFHRAMKYDK DEVDLLIVIG SSLKVRPVAL
IPSSIPHEVP QILINREPLP HLHFDVELLG DCDVIINELC HRLGGEYAKL
CCNPVKLSEI TEKPPRTQKE LAYLSELPPT PLHVSEDSSS PERTSPPDSS
VIVTLLDQAA KSNDDLDVSE SKGCMEEKPQ EVQTSRNVES IAEQMENPDL
KNVGSSTGEK NERTSVAGTV RKCWPNRVAK EQISRRLDGN QYLFLPPNRY
IFHGAEVYSD SEDDVLSSSS CGSNSDSGTC QSPSLEEPME DESEIEEFYN
GLEDEPDVPE RAGGAGFGTD GDDQEAINEA ISVKQEVTDM NYPSNKS
UCSC uc001jnd.3; human. [Q96EB6-1];

Category

Human SIRT1 ELISA Kit