Human STAT2 ELISA Kit

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  • Alternative name

    Human P113 ELISA Kit;Human IMD44 ELISA Kit;Human ISGF-3 ELISA Kit;Human STAT113 ELISA Kit;Human signal transducer and activator of transcription 2 ELISA Kit;Human interferon alpha induced transcriptional activator ELISA Kit;Human signal transducer and activator of transcription 2, 113kDa ELISA Kit;

  • Catalog
    E035259
  • species
    Human
  • GeneSTAT2
  • Standard CurveHuman STAT2 ELISA Kit
  • Other Species Mouse Stat2 ELISA KitPorcine STAT2 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman STAT2 ELISA Kit allows for the in vitro quantitative determination of STAT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: STAT2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-1. Standards or samples are then added to the microtiter plate wells and STAT2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of STAT2 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for STAT2 are added to each well to "sandwich" the STAT2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain STAT2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STAT2 concentration in each sample is interpolated from this standard curve.
  • Human Signal transducer and activator of transcription 2 Protein information
  • Uniprot ID STAT2_HUMAN
  • Uniprot AC P52630; B4DLC7; G3V2M6; Q16430; Q16431; Q9UDL4;
  • UniGene Hs.530595;
  • GeneID 6773
  • KEGG hsa:6773;
  • Human Signal transducer and activator of transcription 2 Protein SEQUENCE
  • SEQUENCE 851 AA; 97916 MW; E4C74674CB7A3215 CRC64;

    MAQWEMLQNL DSPFQDQLHQ LYSHSLLPVD IRQYLAVWIE DQNWQEAALG

    SDDSKATMLF FHFLDQLNYE CGRCSQDPES LLLQHNLRKF CRDIQPFSQD

    PTQLAEMIFN LLLEEKRILI QAQRAQLEQG EPVLETPVES QQHEIESRIL

    DLRAMMEKLV KSISQLKDQQ DVFCFRYKIQ AKGKTPSLDP HQTKEQKILQ

    ETLNELDKRR KEVLDASKAL LGRLTTLIEL LLPKLEEWKA QQQKACIRAP

    IDHGLEQLET WFTAGAKLLF HLRQLLKELK GLSCLVSYQD DPLTKGVDLR

    NAQVTELLQR LLHRAFVVET QPCMPQTPHR PLILKTGSKF TVRTRLLVRL

    QEGNESLTVE VSIDRNPPQL QGFRKFNILT SNQKTLTPEK GQSQGLIWDF

    GYLTLVEQRS GGSGKGSNKG PLGVTEELHI ISFTVKYTYQ GLKQELKTDT

    LPVVIISNMN QLSIAWASVL WFNLLSPNLQ NQQFFSNPPK APWSLLGPAL

    SWQFSSYVGR GLNSDQLSML RNKLFGQNCR TEDPLLSWAD FTKRESPPGK

    LPFWTWLDKI LELVHDHLKD LWNDGRIMGF VSRSQERRLL KKTMSGTFLL

    RFSESSEGGI TCSWVEHQDD DKVLIYSVQP YTKEVLQSLP LTEIIRHYQL

    LTEENIPENP LRFLYPRIPR DEAFGCYYQE KVNLQERRKY LKHRLIVVSN

    RQVDELQQPL ELKPEPELES LELELGLVPE PELSLDLEPL LKAGLDLGPE

    LESVLESTLE PVIEPTLCMV SQTVPEPDQG PVSQPVPEPD LPCDLRHLNT

    EPMEIFRNCV KIEEIMPNGD PLLAGQNTVD EVYVSRPSHF YTDGPLMPSD

    F

  • UCSC uc001sld.4; human. [P52630-3];


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