Human SIRPB1 ELISA Kit

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  • Alternative name

    Human SIRP-beta-1 isoform 3 ELISA Kit;Human CD172b ELISA Kit;Human SIRP-BETA-1 ELISA Kit;Human signal regulatory protein beta 1 ELISA Kit;Human signal-regulatory protein beta-1 ELISA Kit;Human CD172 antigen-like family member B ELISA Kit;

  • Catalog
    E035247
  • species
    Human
  • GeneSIRPB1
  • Standard CurveHuman SIRPB1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Signal Regulatory Protein Beta 1 (SIRPb1). No significant cross-reactivity or interference between Signal Regulatory Protein Beta 1 (SIRPb1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.109ng/mL.
  • Intended UseHuman SIRPB1 ELISA Kit allows for the in vitro quantitative determination of SIRPB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Regulatory Protein Beta 1 (SIRPb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Regulatory Protein Beta 1 (SIRPb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Regulatory Protein Beta 1 (SIRPb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Regulatory Protein Beta 1 (SIRPb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Signal-regulatory protein beta-1 isoform 3 Protein information
  • Uniprot ID SIRBL_HUMAN
  • Uniprot AC Q5TFQ8; Q7Z3B9;
  • UniGene Hs.664861;
  • GeneID 10326
  • KEGG hsa:10326;
  • Human Signal-regulatory protein beta-1 isoform 3 Protein SEQUENCE
  • SEQUENCE 398 AA; 43359 MW; 7F3B930235BD556C CRC64;

    MPVPASWPHL PSPFLLMTLL LGRLTGVAGE EELQVIQPDK SISVAAGESA

    TLHCTVTSLI PVGPIQWFRG AGPGRELIYN QKEGHFPRVT TVSDLTKRNN

    MDFSIRISNI TPADAGTYYC VKFRKGSPDH VEFKSGAGTE LSVRAKPSAP

    VVSGPAARAT PQHTVSFTCE SHGFSPRDIT LKWFKNGNEL SDFQTNVDPA

    GDSVSYSIHS TAKVVLTRED VHSQVICEVA HVTLQGDPLR GTANLSETIR

    VPPTLEVTQQ PVRAENQVNV TCQVRKFYPQ RLQLTWLENG NVSRTETAST

    LTENKDGTYN WMSWLLVNVS AHRDDVKLTC QVEHDGQPAV SKSHDLKVSA

    HPKEQGSNTA PGPALASAAP LLIAFLLGPK VLLVVGVSVI YVYWKQKA

  • UCSC uc002wfl.5; human. [Q5TFQ8-1];


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