Human SIGMAR1 ELISA Kit

Alternative name
Human aging-associated gene 8 protein ELISA Kit;Human SR31747-binding protein ELISA Kit;Human SR-BP ELISA Kit;Human sigma 1-type opioid receptor ELISA Kit;Human SIG-1R ELISA Kit;Human Sigma1-receptor ELISA Kit;Human sigma1R ELISA Kit;Human hSigmaR1 ELISA Kit;Human OPRS1 ELISA Kit;Human SRBP ELISA Kit;Human ALS16 ELISA Kit;Human DSMA2 ELISA Kit;Human SR-BP1 ELISA Kit;Human sigma non-opioid intracellular receptor 1 ELISA Kit;Human SR31747 binding protein 1 ELISA Kit;
Catalog
E035220
species
Human
GeneSIGMAR1
SpecificityThis assay has high sensitivity and excellent specificity for detection of SIGMAR1. No significant cross-reactivity or interference between SIGMAR1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SIGMAR1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman SIGMAR1 ELISA Kit allows for the in vitro quantitative determination of SIGMAR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: SIGMAR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SIGMAR1 antibody and an SIGMAR1-HRP conjugate. The assay sample and buffer are incubated together with SIGMAR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SIGMAR1 concentration since SIGMAR1 from samples and SIGMAR1-HRP conjugate compete for the anti-SIGMAR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SIGMAR1 from the sample, fewer sites are left to bind SIGMAR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SIGMAR1 concentration in each sample is interpolated from this standard curve.
Human Sigma non-opioid intracellular receptor 1 Protein information
Uniprot ID SGMR1_HUMAN
Uniprot AC Q7Z653; Q8N7H3; Q9NYX0;
UniGene Hs.522087;
GeneID 10280
KEGG hsa:10280;
Human Sigma non-opioid intracellular receptor 1 Protein SEQUENCE
SEQUENCE 223 AA; 25128 MW; 0C498636BEB1C443 CRC64;
MQWAVGRRWA WAALLLAVAA VLTQVVWLWL GTQSFVFQRE EIAQLARQYA
GLDHELAFSR LIVELRRLHP GHVLPDEELQ WVFVNAGGWM GAMCLLHASL
SEYVLLFGTA LGSRGHSGRY WAEISDTIIS GTFHQWREGT TKSEVFYPGE
TVVHGPGEAT AVEWGPNTWM VEYGRGVIPS TLAFALADTV FSTQDFLTLF
YTLRSYARGL RLELTTYLFG QDP
UCSC uc003zvb.5; human. [Q99720-1];

Human SIGMAR1 ELISA Kit

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