Human SIGMAR1 ELISA Kit

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  • Alternative name

    Human aging-associated gene 8 protein ELISA Kit;Human SR31747-binding protein ELISA Kit;Human SR-BP ELISA Kit;Human sigma 1-type opioid receptor ELISA Kit;Human SIG-1R ELISA Kit;Human Sigma1-receptor ELISA Kit;Human sigma1R ELISA Kit;Human hSigmaR1 ELISA Kit;Human OPRS1 ELISA Kit;Human SRBP ELISA Kit;Human ALS16 ELISA Kit;Human DSMA2 ELISA Kit;Human SR-BP1 ELISA Kit;Human sigma non-opioid intracellular receptor 1 ELISA Kit;Human SR31747 binding protein 1 ELISA Kit;

  • Catalog
    E035220
  • species
    Human
  • GeneSIGMAR1
  • Standard CurveHuman SIGMAR1 ELISA Kit
  • Other Species Mouse Sigmar1 ELISA KitBovine SIGMAR1 ELISA KitGuinea Pig SIGMAR1 ELISA KitChicken SIGMAR1 ELISA KitRat Sigmar1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SIGMAR1. No significant cross-reactivity or interference between SIGMAR1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SIGMAR1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman SIGMAR1 ELISA Kit allows for the in vitro quantitative determination of SIGMAR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: SIGMAR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SIGMAR1 antibody and an SIGMAR1-HRP conjugate. The assay sample and buffer are incubated together with SIGMAR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SIGMAR1 concentration since SIGMAR1 from samples and SIGMAR1-HRP conjugate compete for the anti-SIGMAR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SIGMAR1 from the sample, fewer sites are left to bind SIGMAR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SIGMAR1 concentration in each sample is interpolated from this standard curve.
  • Human Sigma non-opioid intracellular receptor 1 Protein information
  • Uniprot ID SGMR1_HUMAN
  • Uniprot AC Q7Z653; Q8N7H3; Q9NYX0;
  • UniGene Hs.522087;
  • GeneID 10280
  • KEGG hsa:10280;
  • Human Sigma non-opioid intracellular receptor 1 Protein SEQUENCE
  • SEQUENCE 223 AA; 25128 MW; 0C498636BEB1C443 CRC64;

    MQWAVGRRWA WAALLLAVAA VLTQVVWLWL GTQSFVFQRE EIAQLARQYA

    GLDHELAFSR LIVELRRLHP GHVLPDEELQ WVFVNAGGWM GAMCLLHASL

    SEYVLLFGTA LGSRGHSGRY WAEISDTIIS GTFHQWREGT TKSEVFYPGE

    TVVHGPGEAT AVEWGPNTWM VEYGRGVIPS TLAFALADTV FSTQDFLTLF

    YTLRSYARGL RLELTTYLFG QDP

  • UCSC uc003zvb.5; human. [Q99720-1];


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