Human ALOX15B ELISA Kit

Alternative name
Human 15-LOX-B ELISA Kit;Human 15-lipoxygenase 2 ELISA Kit;Human 15-LOX-2 ELISA Kit;Human arachidonate 15-lipoxygenase type II ELISA Kit;Human linoleate 13-lipoxygenase 15-LOb ELISA Kit;Human arachidonate 15-lipoxygenase, type B ELISA Kit;Human arachidonate 15-lipoxygenase B ELISA Kit;Human 15S-lipoxygenase ELISA Kit;Human arachidonate 15-lipoxygenase 2 ELISA Kit;Human arachidonate 15-lipoxygenase, second type ELISA Kit;Human arachidonate omega(6) lipoxygenase ELISA Kit;
Catalog
E003522
species
Human
GeneALOX15B
Standard Curve
Other Species Mouse ALOX15B ELISA Kit Rat Alox15b ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ALOX15B. No significant cross-reactivity or interference between ALOX15B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALOX15B and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ALOX15B ELISA Kit allows for the in vitro quantitative determination of ALOX15B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: ALOX15B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALOX15B antibody and an ALOX15B-HRP conjugate. The assay sample and buffer are incubated together with ALOX15B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALOX15B concentration since ALOX15B from samples and ALOX15B-HRP conjugate compete for the anti-ALOX15B antibody binding site. Since the number of sites is limited, as more sites are occupied by ALOX15B from the sample, fewer sites are left to bind ALOX15B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALOX15B concentration in each sample is interpolated from this standard curve.
Human Arachidonate 15-lipoxygenase B Protein information
Uniprot ID LX15B_HUMAN
Uniprot AC O15296; D3DTR2; Q8IYQ2; Q8TEV3; Q8TEV4; Q8TEV5; Q8TEV6; Q9UKM4;
UniGene Hs.111256;
GeneID 247
KEGG hsa:247;
Human Arachidonate 15-lipoxygenase B Protein SEQUENCE
SEQUENCE 676 AA; 75857 MW; 4F641DF2F9D492C6 CRC64;
MAEFRVRVST GEAFGAGTWD KVSVSIVGTR GESPPLPLDN LGKEFTAGAE
EDFQVTLPED VGRVLLLRVH KAPPVLPLLG PLAPDAWFCR WFQLTPPRGG
HLLFPCYQWL EGAGTLVLQE GTAKVSWADH HPVLQQQRQE ELQARQEMYQ
WKAYNPGWPH CLDEKTVEDL ELNIKYSTAK NANFYLQAGS AFAEMKIKGL
LDRKGLWRSL NEMKRIFNFR RTPAAEHAFE HWQEDAFFAS QFLNGLNPVL
IRRCHYLPKN FPVTDAMVAS VLGPGTSLQA ELEKGSLFLV DHGILSGIQT
NVINGKPQFS AAPMTLLYQS PGCGPLLPLA IQLSQTPGPN SPIFLPTDDK
WDWLLAKTWV RNAEFSFHEA LTHLLHSHLL PEVFTLATLR QLPHCHPLFK
LLIPHTRYTL HINTLARELL IVPGQVVDRS TGIGIEGFSE LIQRNMKQLN
YSLLCLPEDI RTRGVEDIPG YYYRDDGMQI WGAVERFVSE IIGIYYPSDE
SVQDDRELQA WVREIFSKGF LNQESSGIPS SLETREALVQ YVTMVIFTCS
AKHAAVSAGQ FDSCAWMPNL PPSMQLPPPT SKGLATCEGF IATLPPVNAT
CDVILALWLL SKEPGDQRPL GTYPDEHFTE EAPRRSIATF QSRLAQISRG
IQERNQGLVL PYTYLDPPLI ENSVSI
UCSC uc002gju.4; human. [O15296-1];

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Human ALOX15B ELISA Kit