Human SIGLEC8 ELISA Kit

Alternative name
Human SIGLEC-8 ELISA Kit;Human sialoadhesin family member 2 ELISA Kit;Human SAF-2 ELISA Kit;Human SAF2 ELISA Kit;Human SIGLEC8L ELISA Kit;Human sialic acid binding Ig like lectin 8 ELISA Kit;Human sialic acid-binding Ig-like lectin 8 ELISA Kit;Human CDw329 ELISA Kit;
Catalog
E035176
species
Human
GeneSIGLEC8
Standard Curve
Other Species Mouse SIGLEC8 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8). No significant cross-reactivity or interference between Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman SIGLEC8 ELISA Kit allows for the in vitro quantitative determination of SIGLEC8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Sialic acid-binding Ig-like lectin 8 Protein information
Uniprot ID SIGL8_HUMAN
Uniprot AC Q9NYZ4; Q7Z728;
UniGene Hs.447899;
GeneID 27181
KEGG hsa:27181;
Human Sialic acid-binding Ig-like lectin 8 Protein SEQUENCE
SEQUENCE 499 AA; 54042 MW; 086EFF989B74123C CRC64;
MLLLLLLLPL LWGTKGMEGD RQYGDGYLLQ VQELVTVQEG LCVHVPCSFS
YPQDGWTDSD PVHGYWFRAG DRPYQDAPVA TNNPDREVQA ETQGRFQLLG
DIWSNDCSLS IRDARKRDKG SYFFRLERGS MKWSYKSQLN YKTKQLSVFV
TALTHRPDIL ILGTLESGHS RNLTCSVPWA CKQGTPPMIS WIGASVSSPG
PTTARSSVLT LTPKPQDHGT SLTCQVTLPG TGVTTTSTVR LDVSYPPWNL
TMTVFQGDAT ASTALGNGSS LSVLEGQSLR LVCAVNSNPP ARLSWTRGSL
TLCPSRSSNP GLLELPRVHV RDEGEFTCRA QNAQGSQHIS LSLSLQNEGT
GTSRPVSQVT LAAVGGAGAT ALAFLSFCII FIIVRSCRKK SARPAAGVGD
TGMEDAKAIR GSASQGPLTE SWKDGNPLKK PPPAVAPSSG EEGELHYATL
SFHKVKPQDP QGQEATDSEY SEIKIHKRET AETQACLRNH NPSSKEVRG
UCSC uc002pwt.3; human. [Q9NYZ4-1];

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Human SIGLEC8 ELISA Kit

Human SIGLEC-8 ELISA Kit;Human sialoadhesin family member 2 ELISA Kit;Human SAF-2 ELISA Kit;Human SAF2 ELISA Kit;Human SIGLEC8L ELISA Kit;Human sialic acid binding Ig like lectin 8 ELISA Kit;Human sialic acid-binding Ig-like lectin 8 ELISA Kit;Human CDw329 ELISA Kit;