Human SIGLEC12 ELISA Kit

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  • Alternative name

    Human Siglec-12 ELISA Kit;Human sialic acid-binding Ig-like lectin-like 1 ELISA Kit;Human Siglec-L1 ELISA Kit;Human SIGLECL1 ELISA Kit;Human SLG ELISA Kit;Human S2V ELISA Kit;Human Siglec-XII ELISA Kit;Human sialic acid binding Ig like lectin 12 (gene/pseudogene) ELISA Kit;Human sialic acid-binding Ig-like lectin 12 ELISA Kit;Human SIGLEC-like 1 ELISA Kit;Human sialic acid binding Ig-like lectin 12 ELISA Kit;

  • Catalog
    E035166
  • species
    Human
  • GeneSIGLEC12
  • Standard CurveHuman SIGLEC12 ELISA Kit
  • Other Species Mouse Siglec12 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman SIGLEC12 ELISA Kit allows for the in vitro quantitative determination of SIGLEC12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Principle of the Assay: SIGLEC12 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SIGLEC12. Standards or samples are then added to the microtiter plate wells and SIGLEC12 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SIGLEC12 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for SIGLEC12 are added to each well to "sandwich" the SIGLEC12 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SIGLEC12 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SIGLEC12 concentration in each sample is interpolated from this standard curve.
  • Human Sialic acid-binding Ig-like lectin 12 Protein information
  • Uniprot ID SIG12_HUMAN
  • Uniprot AC Q96PQ1; Q8IYH7;
  • UniGene Hs.249741;
  • GeneID 89858
  • KEGG hsa:89858;
  • Human Sialic acid-binding Ig-like lectin 12 Protein SEQUENCE
  • SEQUENCE 595 AA; 64984 MW; D05662176274C5C3 CRC64;

    MLLLLLLLPP LLCGRVGAKE QKDYLLTMQK SVTVQEGLCV SVLCSFSYPQ

    NGWTASDPVH GYWFRAGDHV SRNIPVATNN PARAVQEETR DRFHLLGDPQ

    NKDCTLSIRD TRESDAGTYV FCVERGNMKW NYKYDQLSVN VTASQDLLSR

    YRLEVPESVT VQEGLCVSVP CSVLYPHYNW TASSPVYGSW FKEGADIPWD

    IPVATNTPSG KVQEDTHGRF LLLGDPQTNN CSLSIRDARK GDSGKYYFQV

    ERGSRKWNYI YDKLSVHVTA LTHMPTFSIP GTLESGHPRN LTCSVPWACE

    QGTPPTITWM GASVSSLDPT ITRSSMLSLI PQPQDHGTSL TCQVTLPGAG

    VTMTRAVRLN ISYPPQNLTM TVFQGDGTAS TTLRNGSALS VLEGQSLHLV

    CAVDSNPPAR LSWTWGSLTL SPSQSSNLGV LELPRVHVKD EGEFTCRAQN

    PLGSQHISLS LSLQNEYTGK MRPISGVTLG AFGGAGATAL VFLYFCIIFV

    VVRSCRKKSA RPAVGVGDTG MEDANAVRGS ASQGPLIESP ADDSPPHHAP

    PALATPSPEE GEIQYASLSF HKARPQYPQE QEAIGYEYSE INIPK

  • UCSC uc002pww.3; human. [Q96PQ1-1];



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