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Human SIAE ELISA Kit

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  • Alternative name

    Human H-Lse ELISA Kit;Human Sialic acid-specific 9-O-acetylesterase ELISA Kit;Human YSG2 ELISA Kit;Human AIS6 ELISA Kit;Human CSE-C ELISA Kit;Human CSEC ELISA Kit;Human LSE ELISA Kit;Human sialic acid acetylesterase ELISA Kit;Human sialate O-acetylesterase ELISA Kit;Human cytosolic sialic acid 9-O-acetylesterase homolog ELISA Kit;Human sialic acid-specific acetylesterase II ELISA Kit;
  • Catalog
    E035160
  • species
    Human
  • GeneSIAE
  • Standard CurveHuman SIAE ELISA Kit
  • Other Species Mouse Siae ELISA KitRat Siae ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Sialic Acid Acetylesterase (SIAE). No significant cross-reactivity or interference between Sialic Acid Acetylesterase (SIAE) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman SIAE ELISA Kit allows for the in vitro quantitative determination of SIAE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sialic Acid Acetylesterase (SIAE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sialic Acid Acetylesterase (SIAE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sialic Acid Acetylesterase (SIAE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sialic Acid Acetylesterase (SIAE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Sialate O-acetylesterase Protein information
  • Uniprot ID SIAE_HUMAN
  • Uniprot AC Q9HAT2; B3KPB0; Q8IUT9; Q9HAU7; Q9NT71;
  • UniGene Hs.10056;
  • GeneID 54414
  • KEGG hsa:54414;
  • Human Sialate O-acetylesterase Protein SEQUENCE

  • SEQUENCE 523 AA; 58315 MW; B72CF69636DBFED8 CRC64;

    MVAPGLVLGL VLPLILWADR SAGIGFRFAS YINNDMVLQK EPAGAVIWGF

    GTPGATVTVT LRQGQETIMK KVTSVKAHSD TWMVVLDPMK PGGPFEVMAQ

    QTLEKINFTL RVHDVLFGDV WLCSGQSNMQ MTVLQIFNAT RELSNTAAYQ

    SVRILSVSPI QAEQELEDLV AVDLQWSKPT SENLGHGYFK YMSAVCWLFG

    RHLYDTLQYP IGLIASSWGG TPIEAWSSGR SLKACGVPKQ GSIPYDSVTG

    PSKHSVLWNA MIHPLCNMTL KGVVWYQGES NINYNTDLYN CTFPALIEDW

    RETFHRGSQG QTERFFPFGL VQLSSDLSKK SSDDGFPQIR WHQTADFGYV

    PNPKMPNTFM AVAMDLCDRD SPFGSIHPRD KQTVAYRLHL GARALAYGEK

    NLTFEGPLPE KIELLAHKGL LNLTYYQQIQ VQKKDNKIFE ISCCSDHRCK

    WLPASMNTVS TQSLTLAIDS CHGTVVALRY AWTTWPCEYK QCPLYHPSSA

    LPAPPFIAFI TDQGPGHQSN VAK

  • UCSC uc001qan.4; human. [Q9HAT2-1];


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