Human SF1 ELISA Kit

Alternative name
Human mammalian branch point-binding protein ELISA Kit;Human BBP ELISA Kit;Human MBBP ELISA Kit;Human transcription factor ZFM1 ELISA Kit;Human zinc finger gene in MEN1 locus ELISA Kit;Human zinc finger protein 162 ELISA Kit;Human ZFM1 ELISA Kit;Human ZNF162 ELISA Kit;Human D11S636 ELISA Kit;Human ZCCHC25 ELISA Kit;Human splicing factor 1 ELISA Kit;
Catalog
E035037
species
Human
GeneSF1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human SF1. No significant cross-reactivity or interference between Human SF1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human SF1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman SF1 ELISA Kit allows for the in vitro quantitative determination of SF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SF1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SF1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human SF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human SF1. You can calculate the concentration of Human SF1 in the samples by comparing the OD of the samples to the standard curve.
Human Splicing factor 1 Protein information
Uniprot ID SF01_HUMAN
Uniprot AC Q92745; Q969H7; Q9BW01; Q9UEI0;
UniGene Hs.502829;
GeneID 7536
KEGG hsa:7536;
Human Splicing factor 1 Protein SEQUENCE
SEQUENCE 639 AA; 68330 MW; EEBC6A02B29DAE4D CRC64;
MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER
AYIVQLQIED LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR
TRKKLEEERH NLITEMVALN PDFKPPADYK PPATRVSDKV MIPQDEYPEI
NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK GSVKEGKVGR KDGQMLPGED
EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR KMQLRELARL
NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP
QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA
NNPPPPSLMS TTQSRPPWMN SGPSESRPYH GMHGGGPGGP GGGPHSFPHP
LPSLTGGHGG HPMQHNPNGP PPPWMQPPPP PMNQGPHPPG HHGPPPMDQY
LGSTPVGSGV YRLHQGKGMM PPPPMGMMPP PPPPPSGQPP PPPSGPLPPW
QQQQQQPPPP PPPSSSMASS TPLPWQQNTT TTTTSAGTGS IPPWQQQQAA
AAASPGAPQM QGNPTMVPLP PGVQPPLPPG APPPPPPPPP GSAGMMYAPP
PPPPPPMDPS NFVTMMGMGV AGMPPFGMPP APPPPPPQN
UCSC uc001oaz.3; human. [Q15637-1];

Category

Human SF1 ELISA Kit