Human SHBG ELISA Kit

Alternative name
Human SHBG ELISA Kit;Human sex steroid-binding protein ELISA Kit;Human SBP ELISA Kit;Human testis-specific androgen-binding protein ELISA Kit;Human ABP ELISA Kit;Human testosterone-estradiol-binding globulin ELISA Kit;Human TEBG ELISA Kit;Human testosterone-estrogen-binding globulin ELISA Kit;Human sex hormone binding globulin ELISA Kit;Human sex hormone-binding globulin ELISA Kit;Human testosterone-binding beta-globulin ELISA Kit;
Catalog
E035028
species
Human
GeneSHBG
SpecificityThis assay has high sensitivity and excellent specificity for detection of SHBG. No significant cross-reactivity or interference between SHBG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SHBG and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 nmol/mL.
Intended UseHuman SHBG ELISA Kit allows for the in vitro quantitative determination of SHBG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: SHBG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SHBG antibody and an SHBG-HRP conjugate. The assay sample and buffer are incubated together with SHBG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SHBG concentration since SHBG from samples and SHBG-HRP conjugate compete for the anti-SHBG antibody binding site. Since the number of sites is limited, as more sites are occupied by SHBG from the sample, fewer sites are left to bind SHBG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SHBG concentration in each sample is interpolated from this standard curve.
Human Sex hormone-binding globulin Protein information
Uniprot ID SHBG_HUMAN
Uniprot AC Q6ISD2;
UniGene Hs.632235;
GeneID 6462
KEGG hsa:6462;
Human Sex hormone-binding globulin Protein SEQUENCE
SEQUENCE 402 AA; 43779 MW; 5A3B1885E4E7A460 CRC64;
MESRGPLATS RLLLLLLLLL LRHTRQGWAL RPVLPTQSAH DPPAVHLSNG
PGQEPIAVMT FDLTKITKTS SSFEVRTWDP EGVIFYGDTN PKDDWFMLGL
RDGRPEIQLH NHWAQLTVGA GPRLDDGRWH QVEVKMEGDS VLLEVDGEEV
LRLRQVSGPL TSKRHPIMRI ALGGLLFPAS NLRLPLVPAL DGCLRRDSWL
DKQAEISASA PTSLRSCDVE SNPGIFLPPG TQAEFNLRDI PQPHAEPWAF
SLDLGLKQAA GSGHLLALGT PENPSWLSLH LQDQKVVLSS GSGPGLDLPL
VLGLPLQLKL SMSRVVLSQG SKMKALALPP LGLAPLLNLW AKPQGRLFLG
ALPGEDSSTS FCLNGLWAQG QRLDVDQALN RSHEIWTHSC PQSPGNGTDA
SH
UCSC uc002gie.4; human. [P04278-1];

Category

Human SHBG ELISA Kit