Human SHBG ELISA Kit

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  • Alternative name

    Human SHBG ELISA Kit;Human sex steroid-binding protein ELISA Kit;Human SBP ELISA Kit;Human testis-specific androgen-binding protein ELISA Kit;Human ABP ELISA Kit;Human testosterone-estradiol-binding globulin ELISA Kit;Human TEBG ELISA Kit;Human testosterone-estrogen-binding globulin ELISA Kit;Human sex hormone binding globulin ELISA Kit;Human sex hormone-binding globulin ELISA Kit;Human testosterone-binding beta-globulin ELISA Kit;

  • Catalog
    E035028
  • species
    Human
  • GeneSHBG
  • Standard CurveHuman SHBG ELISA Kit
  • Other Species Mouse Shbg ELISA KitRabbit SHBG ELISA KitRat Shbg ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SHBG. No significant cross-reactivity or interference between SHBG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SHBG and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 nmol/mL.
  • Intended UseHuman SHBG ELISA Kit allows for the in vitro quantitative determination of SHBG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: SHBG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SHBG antibody and an SHBG-HRP conjugate. The assay sample and buffer are incubated together with SHBG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SHBG concentration since SHBG from samples and SHBG-HRP conjugate compete for the anti-SHBG antibody binding site. Since the number of sites is limited, as more sites are occupied by SHBG from the sample, fewer sites are left to bind SHBG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SHBG concentration in each sample is interpolated from this standard curve.
  • Human Sex hormone-binding globulin Protein information
  • Uniprot ID SHBG_HUMAN
  • Uniprot AC Q6ISD2;
  • UniGene Hs.632235;
  • GeneID 6462
  • KEGG hsa:6462;
  • Human Sex hormone-binding globulin Protein SEQUENCE
  • SEQUENCE 402 AA; 43779 MW; 5A3B1885E4E7A460 CRC64;

    MESRGPLATS RLLLLLLLLL LRHTRQGWAL RPVLPTQSAH DPPAVHLSNG

    PGQEPIAVMT FDLTKITKTS SSFEVRTWDP EGVIFYGDTN PKDDWFMLGL

    RDGRPEIQLH NHWAQLTVGA GPRLDDGRWH QVEVKMEGDS VLLEVDGEEV

    LRLRQVSGPL TSKRHPIMRI ALGGLLFPAS NLRLPLVPAL DGCLRRDSWL

    DKQAEISASA PTSLRSCDVE SNPGIFLPPG TQAEFNLRDI PQPHAEPWAF

    SLDLGLKQAA GSGHLLALGT PENPSWLSLH LQDQKVVLSS GSGPGLDLPL

    VLGLPLQLKL SMSRVVLSQG SKMKALALPP LGLAPLLNLW AKPQGRLFLG

    ALPGEDSSTS FCLNGLWAQG QRLDVDQALN RSHEIWTHSC PQSPGNGTDA

    SH

  • UCSC uc002gie.4; human. [P04278-1];


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