Human SESN3 ELISA Kit

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  • Alternative name

    Human SEST3 ELISA Kit;Human sestrin 3 ELISA Kit;Human sestrin-3 ELISA Kit;

  • Catalog
    E034977
  • species
    Human
  • GeneSESN3
  • Standard CurveHuman SESN3 ELISA Kit
  • Other Species Mouse Sesn3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SESN3. No significant cross-reactivity or interference between SESN3 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of SESN3 is typically less than 0.058ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
  • Intended UseHuman SESN3 ELISA Kit allows for the in vitro quantitative determination of SESN3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SESN3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SESN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SESN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SESN3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Sestrin-3 Protein information
  • Uniprot ID SESN3_HUMAN
  • Uniprot AC P58005; B7Z7P9; Q96AD1;
  • UniGene Hs.120633;
  • GeneID 143686
  • KEGG hsa:143686;
  • Human Sestrin-3 Protein SEQUENCE
  • SEQUENCE 492 AA; 57291 MW; 0B165C6BAE5A49CF CRC64;

    MNRGGGSPSA AANYLLCTNC RKVLRKDKRI RVSQPLTRGP SAFIPEKEVV

    QANTVDERTN FLVEEYSTSG RLDNITQVMS LHTQYLESFL RSQFYMLRMD

    GPLPLPYRHY IAIMAAARHQ CSYLINMHVD EFLKTGGIAE WLNGLEYVPQ

    RLKNLNEINK LLAHRPWLIT KEHIQKLVKT GENNWSLPEL VHAVVLLAHY

    HALASFVFGS GINPERDPEI SNGFRLISVN NFCVCDLAND NNIENASLSG

    SNFGIVDSLS ELEALMERMK RLQEEREDEE ASQEEMSTRF EKEKKESLFV

    VSGDTFHSFP HSDFEDDMII TSDVSRYIED PGFGYEDFAR RGEEHLPTFR

    AQDYTWENHG FSLVNRLYSD IGHLLDEKFR MVYNLTYNTM ATHEDVDTTM

    LRRALFNYVH CMFGIRYDDY DYGEVNQLLE RSLKVYIKTV TCYPERTTKR

    MYDSYWRQFK HSEKVHVNLL LMEARMQAEL LYALRAITRH LT

  • UCSC uc001pfj.5; human. [P58005-1];


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