Human SAA3 ELISA Kit

Contact Us
  • Alternative name

    Serum amyloid A-3 protein ELISA KIT; Saa3 ELISA KIT;

  • Catalog
    E034933
  • species
    Human
  • GeneSAA3
  • Other Species Mouse Saa3 ELISA KitRabbit SAA3 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ug/mL.
  • Intended UseHuman SAA3 ELISA Kit allows for the in vitro quantitative determination of SAA3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Principle of the Assay: SAA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SAA3 antibody and an SAA3-HRP conjugate. The assay sample and buffer are incubated together with SAA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SAA3 concentration since SAA3 from samples and SAA3-HRP conjugate compete for the anti-SAA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by SAA3 from the sample, fewer sites are left to bind SAA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAA3 concentration in each sample is interpolated from this standard curve.



Inquiry About Human SAA3 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!