Human BSA ELISA Kit

Contact Us
  • Alternative name

    Serum albumin ELISA KIT; ALB ELISA KIT; GIG20 ELISA KIT; GIG42 ELISA KIT; PRO0903 ELISA KIT; PRO1708 ELISA KIT; PRO2044 ELISA KIT; PRO2619 ELISA KIT; PRO2675 ELISA KIT; UNQ696/PRO1341 ELISA KIT

  • Catalog
    E034924
  • species
    Human
  • GeneBSA
  • Other Species Mouse BSA ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman BSA ELISA Kit allows for the in vitro quantitative determination of BSA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Principle of the Assay: BSA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BSA antibody and an BSA-HRP conjugate. The assay sample and buffer are incubated together with BSA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BSA concentration since BSA from samples and BSA-HRP conjugate compete for the anti-BSA antibody binding site. Since the number of sites is limited, as more sites are occupied by BSA from the sample, fewer sites are left to bind BSA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BSA concentration in each sample is interpolated from this standard curve.


Related products with Human BSA ELISA Kit

Inquiry About Human BSA ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!