Human AQP5 ELISA Kit

Alternative name
Human AQP-5 ELISA Kit;Human PPKB ELISA Kit;Human aquaporin 5 ELISA Kit;Human aquaporin-5 ELISA Kit;
Catalog
E003481
species
Human
GeneAQP5
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human AQP5. No significant cross-reactivity or interference between Human AQP5 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.039 ng/ml
Intended UseHuman AQP5 ELISA Kit allows for the in vitro quantitative determination of AQP5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AQP-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for AQP-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain AQP-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AQP-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Aquaporin-5 Protein information
Uniprot ID AQP5_HUMAN
Uniprot AC P55064; Q6FGW8;
UniGene Hs.298023;
GeneID 362
KEGG hsa:362;
Human Aquaporin-5 Protein SEQUENCE
SEQUENCE 265 AA; 28292 MW; 053C10E6A17EAFDA CRC64;
MKKEVCSVAF LKAVFAEFLA TLIFVFFGLG SALKWPSALP TILQIALAFG
LAIGTLAQAL GPVSGGHINP AITLALLVGN QISLLRAFFY VAAQLVGAIA
GAGILYGVAP LNARGNLAVN ALNNNTTQGQ AMVVELILTF QLALCIFAST
DSRRTSPVGS PALSIGLSVT LGHLVGIYFT GCSMNPARSF GPAVVMNRFS
PAHWVFWVGP IVGAVLAAIL YFYLLFPNSL SLSERVAIIK GTYEPDEDWE
EQREERKKTM ELTTR
UCSC uc001rvo.4; human.;

Human AQP5 ELISA Kit

Human AQP-5 ELISA Kit;Human PPKB ELISA Kit;Human aquaporin 5 ELISA Kit;Human aquaporin-5 ELISA Kit;

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