Human HPN ELISA Kit

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  • Alternative name

    Human transmembrane protease serine 1 ELISA Kit;Human TMPRSS1 ELISA Kit;Human hepsin ELISA Kit;Human serine protease hepsin ELISA Kit;Human testicular tissue protein Li 85 ELISA Kit;

  • Catalog
    E034738
  • species
    Human
  • GeneHPN
  • Other Species Mouse Hpn ELISA KitRat Hpn ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.469ng/ml
  • Intended UseHuman HPN ELISA Kit allows for the in vitro quantitative determination of HPN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human HPN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HPN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HPN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human HPN can be calculated.
  • Human Serine protease hepsin catalytic chain Protein information
  • Uniprot ID HEPS_HUMAN
  • Uniprot AC P05981; B2RDS4;
  • UniGene Hs.182385;
  • GeneID 3249
  • KEGG hsa:3249;
  • Human Serine protease hepsin catalytic chain Protein SEQUENCE
  • SEQUENCE 417 AA; 45011 MW; B2086FF661E551D7 CRC64;

    MAQKEGGRTV PCCSRPKVAA LTAGTLLLLT AIGAASWAIV AVLLRSDQEP

    LYPVQVSSAD ARLMVFDKTE GTWRLLCSSR SNARVAGLSC EEMGFLRALT

    HSELDVRTAG ANGTSGFFCV DEGRLPHTQR LLEVISVCDC PRGRFLAAIC

    QDCGRRKLPV DRIVGGRDTS LGRWPWQVSL RYDGAHLCGG SLLSGDWVLT

    AAHCFPERNR VLSRWRVFAG AVAQASPHGL QLGVQAVVYH GGYLPFRDPN

    SEENSNDIAL VHLSSPLPLT EYIQPVCLPA AGQALVDGKI CTVTGWGNTQ

    YYGQQAGVLQ EARVPIISND VCNGADFYGN QIKPKMFCAG YPEGGIDACQ

    GDSGGPFVCE DSISRTPRWR LCGIVSWGTG CALAQKPGVY TKVSDFREWI

    FQAIKTHSEA SGMVTQL

  • UCSC uc002nxq.3; human.;


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