Human SEPT12 ELISA Kit

Alternative name
Human SPGF10 ELISA Kit;Human septin 12 ELISA Kit;Human septin-12 ELISA Kit;Human testicular tissue protein Li 168 ELISA Kit;
Catalog
E034661
species
Human
GeneSEPT12
SpecificityThis assay has high sensitivity and excellent specificity for detection of Septin 12 (SEPT12). No significant cross-reactivity or interference between Septin 12 (SEPT12) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.064ng/mL.
Intended UseHuman SEPT12 ELISA Kit allows for the in vitro quantitative determination of SEPT12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Septin 12 (SEPT12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Septin 12 (SEPT12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Septin 12 (SEPT12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Septin 12 (SEPT12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Septin-12 Protein information
Uniprot ID SEP12_HUMAN
Uniprot AC Q8IYM1; Q0P6B0; Q1PBH0; Q96LL0;
UniGene Hs.126780;
GeneID 124404
KEGG hsa:124404;
Human Septin-12 Protein SEQUENCE
SEQUENCE 358 AA; 40748 MW; 31968F366DEFB4FD CRC64;
MDPLRRSPSP CLSSQPSSPS TPPCEMLGPV GIEAVLDQLK IKAMKMGFEF
NIMVVGQSGL GKSTMVNTLF KSKVWKSNPP GLGVPTPQTL QLHSLTHVIE
EKGVKLKLTV TDTPGFGDQI NNDNCWDPIL GYINEQYEQY LQEEILITRQ
RHIPDTRVHC CVYFVPPTGH CLRPLDIEFL QRLCRTVNVV PVIARADSLT
MEEREAFRRR IQQNLRTHCI DVYPQMCFDE DINDKILNSK LRDRIPFAVV
GADQEHLVNG RCVLGRKTKW GIIEVENMAH CEFPLLRDLL IRSHLQDLKD
ITHNIHYENY RVIRLNESHL LPRGPGWVNL APASPGQLTT PRTFKVCRGA
HDDSDDEF
UCSC uc002cxq.4; human. [Q8IYM1-1];

Category

Human SEPT12 ELISA Kit

Human SPGF10 ELISA Kit;Human septin 12 ELISA Kit;Human septin-12 ELISA Kit;Human testicular tissue protein Li 168 ELISA Kit;