Human SEPT12 ELISA Kit

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  • Alternative name

    Human SPGF10 ELISA Kit;Human septin 12 ELISA Kit;Human septin-12 ELISA Kit;Human testicular tissue protein Li 168 ELISA Kit;

  • Catalog
    E034661
  • species
    Human
  • GeneSEPT12
  • Standard CurveHuman SEPT12 ELISA Kit
  • Other Species Mouse SEPT12 ELISA KitBovine SEPT12 ELISA KitRat Sept12 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Septin 12 (SEPT12). No significant cross-reactivity or interference between Septin 12 (SEPT12) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.064ng/mL.
  • Intended UseHuman SEPT12 ELISA Kit allows for the in vitro quantitative determination of SEPT12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Septin 12 (SEPT12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Septin 12 (SEPT12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Septin 12 (SEPT12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Septin 12 (SEPT12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Septin-12 Protein information
  • Uniprot ID SEP12_HUMAN
  • Uniprot AC Q8IYM1; Q0P6B0; Q1PBH0; Q96LL0;
  • UniGene Hs.126780;
  • GeneID 124404
  • KEGG hsa:124404;
  • Human Septin-12 Protein SEQUENCE
  • SEQUENCE 358 AA; 40748 MW; 31968F366DEFB4FD CRC64;

    MDPLRRSPSP CLSSQPSSPS TPPCEMLGPV GIEAVLDQLK IKAMKMGFEF

    NIMVVGQSGL GKSTMVNTLF KSKVWKSNPP GLGVPTPQTL QLHSLTHVIE

    EKGVKLKLTV TDTPGFGDQI NNDNCWDPIL GYINEQYEQY LQEEILITRQ

    RHIPDTRVHC CVYFVPPTGH CLRPLDIEFL QRLCRTVNVV PVIARADSLT

    MEEREAFRRR IQQNLRTHCI DVYPQMCFDE DINDKILNSK LRDRIPFAVV

    GADQEHLVNG RCVLGRKTKW GIIEVENMAH CEFPLLRDLL IRSHLQDLKD

    ITHNIHYENY RVIRLNESHL LPRGPGWVNL APASPGQLTT PRTFKVCRGA

    HDDSDDEF

  • UCSC uc002cxq.4; human. [Q8IYM1-1];



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