Human SEMA7A ELISA Kit

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  • Alternative name

    Human CDw108 ELISA Kit;Human JMH blood group antigen ELISA Kit;Human john-Milton-Hargen human blood group Ag ELISA Kit;Human semaphorin-K1 ELISA Kit;Human sema K1 ELISA Kit;Human semaphorin-L ELISA Kit;Human sema L ELISA Kit;Human CD108 ELISA Kit;Human SEMAL ELISA Kit;Human H-SEMA-K1 ELISA Kit;Human H-Sema-L ELISA Kit;Human JMH ELISA Kit;Human SEMAK1 ELISA Kit;Human semaphorin 7A (John Milton Hagen blood group) ELISA Kit;Human semaphorin-7A ELISA Kit;Human John Milton Hagen blood group H-Sema K1 ELISA Kit;Human sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group) ELISA Kit;Human sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A ELISA Kit;Human semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) ELISA Kit;

  • Catalog
    E034617
  • species
    Human
  • GeneSEMA7A
  • Standard CurveHuman SEMA7A ELISA Kit
  • Other Species Mouse Sema7a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Semaphorin 7A (SEMA7A). No significant cross-reactivity or interference between Semaphorin 7A (SEMA7A) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.058ng/mL.
  • Intended UseHuman SEMA7A ELISA Kit allows for the in vitro quantitative determination of SEMA7A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 7A (SEMA7A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 7A (SEMA7A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 7A (SEMA7A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semaphorin 7A (SEMA7A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Semaphorin-7A Protein information
  • Uniprot ID SEM7A_HUMAN
  • Uniprot AC O75326; B4DDP7; F5H1S0; Q1XE81; Q1XE82; Q1XE83; Q1XE84; Q3MIY5;
  • UniGene Hs.24640;
  • GeneID 8482
  • KEGG hsa:8482;
  • Human Semaphorin-7A Protein SEQUENCE
  • SEQUENCE 666 AA; 74824 MW; AD3ABE56B5EBE194 CRC64;

    MTPPPPGRAA PSAPRARVPG PPARLGLPLR LRLLLLLWAA AASAQGHLRS

    GPRIFAVWKG HVGQDRVDFG QTEPHTVLFH EPGSSSVWVG GRGKVYLFDF

    PEGKNASVRT VNIGSTKGSC LDKRDCENYI TLLERRSEGL LACGTNARHP

    SCWNLVNGTV VPLGEMRGYA PFSPDENSLV LFEGDEVYST IRKQEYNGKI

    PRFRRIRGES ELYTSDTVMQ NPQFIKATIV HQDQAYDDKI YYFFREDNPD

    KNPEAPLNVS RVAQLCRGDQ GGESSLSVSK WNTFLKAMLV CSDAATNKNF

    NRLQDVFLLP DPSGQWRDTR VYGVFSNPWN YSAVCVYSLG DIDKVFRTSS

    LKGYHSSLPN PRPGKCLPDQ QPIPTETFQV ADRHPEVAQR VEPMGPLKTP

    LFHSKYHYQK VAVHRMQASH GETFHVLYLT TDRGTIHKVV EPGEQEHSFA

    FNIMEIQPFR RAAAIQTMSL DAERRKLYVS SQWEVSQVPL DLCEVYGGGC

    HGCLMSRDPY CGWDQGRCIS IYSSERSVLQ SINPAEPHKE CPNPKPDKAP

    LQKVSLAPNS RYYLSCPMES RHATYSWRHK ENVEQSCEPG HQSPNCILFI

    ENLTAQQYGH YFCEAQEGSY FREAQHWQLL PEDGIMAEHL LGHACALAAS

    LWLGVLPTLT LGLLVH

  • UCSC uc002axv.4; human. [O75326-1];


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