Human AQP1 ELISA Kit

Alternative name
Human AQP-1 ELISA Kit;Human aquaporin-CHIP ELISA Kit;Human urine water channel ELISA Kit;Human water channel protein for red blood cells and kidney proximal tubule ELISA Kit;Human CHIP28 ELISA Kit;Human AQP-CHIP ELISA Kit;Human CO ELISA Kit;Human aquaporin 1 (Colton blood group) ELISA Kit;Human aquaporin-1 ELISA Kit;Human aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) ELISA Kit;Human aquaporin 1, Colton blood group antigen ELISA Kit;Human channel-like integral membrane protein, 28-kDa ELISA Kit;
Catalog
E003460
species
Human
GeneAQP1
SpecificityThis assay has high sensitivity and excellent specificity for detection of AQP-1. No significant cross-reactivity or interference between AQP-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AQP-1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman AQP1 ELISA Kit allows for the in vitro quantitative determination of AQP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This AQP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AQP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||AQP-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AQP-1. Standards or samples are then added to the microtiter plate wells and AQP-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AQP-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AQP-1 are added to each well to "sandwich" the AQP-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AQP-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP-1 concentration in each sample is interpolated from this standard curve.
Human Aquaporin-1 Protein information
Uniprot ID AQP1_HUMAN
Uniprot AC P29972; B5BU39; E7EM69; E9PC21; F5GY19; Q8TBI5; Q8TDC1;
UniGene Hs.76152;
GeneID 358
KEGG hsa:358;
Human Aquaporin-1 Protein SEQUENCE
SEQUENCE 269 AA; 28526 MW; BA204D82FB26352E CRC64;
MASEFKKKLF WRAVVAEFLA TTLFVFISIG SALGFKYPVG NNQTAVQDNV
KVSLAFGLSI ATLAQSVGHI SGAHLNPAVT LGLLLSCQIS IFRALMYIIA
QCVGAIVATA ILSGITSSLT GNSLGRNDLA DGVNSGQGLG IEIIGTLQLV
LCVLATTDRR RRDLGGSAPL AIGLSVALGH LLAIDYTGCG INPARSFGSA
VITHNFSNHW IFWVGPFIGG ALAVLIYDFI LAPRSSDLTD RVKVWTSGQV
EEYDLDADDI NSRVEMKPK
UCSC uc003tbv.3; human. [P29972-1];

Human AQP1 ELISA Kit

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