Human SEMA3C ELISA Kit

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  • Alternative name

    Human Semaphorin-E ELISA Kit;Human sema E ELISA Kit;Human SEMAE ELISA Kit;Human SemE ELISA Kit;Human semaphorin 3C ELISA Kit;Human semaphorin-3C ELISA Kit;Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C ELISA Kit;Human semaphorin E ELISA Kit;

  • Catalog
    E034589
  • species
    Human
  • GeneSEMA3C
  • Standard CurveHuman SEMA3C ELISA Kit
  • Other Species Mouse Sema3c ELISA KitBovine SEMA3C ELISA KitChicken SEMA3C ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SEMA3C. No significant cross-reactivity or interference between SEMA3C and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SEMA3C and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman SEMA3C ELISA Kit allows for the in vitro quantitative determination of SEMA3C , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Intended Uses||This SEMA3C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SEMA3C. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: SEMA3C ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SEMA3C antibody and an SEMA3C-HRP conjugate. The assay sample and buffer are incubated together with SEMA3C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SEMA3C concentration since SEMA3C from samples and SEMA3C-HRP conjugate compete for the anti-SEMA3C antibody binding site. Since the number of sites is limited, as more sites are occupied by SEMA3C from the sample, fewer sites are left to bind SEMA3C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SEMA3C concentration in each sample is interpolated from this standard curve.
  • Human Semaphorin-3C Protein information
  • Uniprot ID SEM3C_HUMAN
  • Uniprot AC Q99985; B4DRL8;
  • UniGene Hs.269109;
  • GeneID 10512
  • KEGG hsa:10512;
  • Human Semaphorin-3C Protein SEQUENCE
  • SEQUENCE 751 AA; 85207 MW; AA68A21FEF20C033 CRC64;

    MAFRTICVLV GVFICSICVK GSSQPQARVY LTFDELRETK TSEYFSLSHH

    PLDYRILLMD EDQDRIYVGS KDHILSLNIN NISQEALSVF WPASTIKVEE

    CKMAGKDPTH GCGNFVRVIQ TFNRTHLYVC GSGAFSPVCT YLNRGRRSED

    QVFMIDSKCE SGKGRCSFNP NVNTVSVMIN EELFSGMYID FMGTDAAIFR

    SLTKRNAVRT DQHNSKWLSE PMFVDAHVIP DGTDPNDAKV YFFFKEKLTD

    NNRSTKQIHS MIARICPNDT GGLRSLVNKW TTFLKARLVC SVTDEDGPET

    HFDELEDVFL LETDNPRTTL VYGIFTTSSS VFKGSAVCVY HLSDIQTVFN

    GPFAHKEGPN HQLISYQGRI PYPRPGTCPG GAFTPNMRTT KEFPDDVVTF

    IRNHPLMYNS IYPIHKRPLI VRIGTDYKYT KIAVDRVNAA DGRYHVLFLG

    TDRGTVQKVV VLPTNNSVSG ELILEELEVF KNHAPITTMK ISSKKQQLYV

    SSNEGVSQVS LHRCHIYGTA CADCCLARDP YCAWDGHSCS RFYPTGKRRS

    RRQDVRHGNP LTQCRGFNLK AYRNAAEIVQ YGVKNNTTFL ECAPKSPQAS

    IKWLLQKDKD RRKEVKLNER IIATSQGLLI RSVQGSDQGL YHCIATENSF

    KQTIAKINFK VLDSEMVAVV TDKWSPWTWA SSVRALPFHP KDIMGAFSHS

    EMQMINQYCK DTRQQHQQGD ESQKMRGDYG KLKALINSRK SRNRRNQLPE

    S

  • UCSC uc003uhj.3; human. [Q99985-1];


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