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Human SEMA3E ELISA Kit

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  • Alternative name

    Human KIAA0331 ELISA Kit;Human SEMAH ELISA Kit;Human M-SEMAH ELISA Kit;Human M-SemaK ELISA Kit;Human coll-5 ELISA Kit;Human semaphorin 3E ELISA Kit;Human semaphorin-3E ELISA Kit;Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E ELISA Kit;
  • Catalog
    E034564
  • species
    Human
  • GeneSEMA3E
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human SEMA3E. No significant cross-reactivity or interference between human SEMA3E and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human SEMA3E is typically less than 5.86 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman SEMA3E ELISA Kit allows for the in vitro quantitative determination of SEMA3E , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA3E has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA3E present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3E is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3E bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Semaphorin-3E Protein information
  • Uniprot ID SEM3E_HUMAN
  • Uniprot AC O15041; B4E1P1; Q75M94; Q75M97;
  • UniGene Hs.528721;
  • GeneID 9723
  • KEGG hsa:9723;
  • Human Semaphorin-3E Protein SEQUENCE

  • SEQUENCE 775 AA; 89228 MW; CD6079C1AE48F779 CRC64;

    MASAGHIITL LLWGYLLELW TGGHTADTTH PRLRLSHKEL LNLNRTSIFH

    SPFGFLDLHT MLLDEYQERL FVGGRDLVYS LSLERISDGY KEIHWPSTAL

    KMEECIMKGK DAGECANYVR VLHHYNRTHL LTCGTGAFDP VCAFIRVGYH

    LEDPLFHLES PRSERGRGRC PFDPSSSFIS TLIGSELFAG LYSDYWSRDA

    AIFRSMGRLA HIRTEHDDER LLKEPKFVGS YMIPDNEDRD DNKVYFFFTE

    KALEAENNAH AIYTRVGRLC VNDVGGQRIL VNKWSTFLKA RLVCSVPGMN

    GIDTYFDELE DVFLLPTRDH KNPVIFGLFN TTSNIFRGHA ICVYHMSSIR

    AAFNGPYAHK EGPEYHWSVY EGKVPYPRPG SCASKVNGGR YGTTKDYPDD

    AIRFARSHPL MYQAIKPAHK KPILVKTDGK YNLKQIAVDR VEAEDGQYDV

    LFIGTDNGIV LKVITIYNQE MESMEEVILE ELQIFKDPVP IISMEISSKR

    QQLYIGSASA VAQVRFHHCD MYGSACADCC LARDPYCAWD GISCSRYYPT

    GTHAKRRFRR QDVRHGNAAQ QCFGQQFVGD ALDKTEEHLA YGIENNSTLL

    ECTPRSLQAK VIWFVQKGRE TRKEEVKTDD RVVKMDLGLL FLRLHKSDAG

    TYFCQTVEHS FVHTVRKITL EVVEEEKVED MFNKDDEEDR HHRMPCPAQS

    SISQGAKPWY KEFLQLIGYS NFQRVEEYCE KVWCTDRKRK KLKMSPSKWK

    YANPQEKKLR SKPEHYRLPR HTLDS

  • UCSC uc003uhy.3; human. [O15041-1];



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