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Alternative name
Selenoprotein P ELISA KIT; SEPP1 ELISA KIT; SELP ELISA KIT; SeP ELISA KIT
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Catalog
E034544
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species
Human
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GeneSEPP1
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Standard Curve
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Other Species
Mouse SEPP1 ELISA Kit
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SpecificityThis assay recognizes recombinant and natural human SeP. No significant cross-reactivity or interference was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseHuman SEPP1 ELISA Kit allows for the in vitro quantitative determination of SEPP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: This immunoassay kit allows for the in vitro quantitative determination of human selenoprotein p,SeP concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to SeP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SeP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SeP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SeP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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