Human SFRP5 ELISA Kit

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  • Alternative name

    Human sFRP-5 ELISA Kit;Human frizzled-related protein 1b ELISA Kit;Human FRP-1b ELISA Kit;Human Secreted apoptosis-related protein 3 ELISA Kit;Human SARP-3 ELISA Kit;Human FRP1B ELISA Kit;Human SARP3 ELISA Kit;Human secreted frizzled related protein 5 ELISA Kit;Human secreted frizzled-related protein 5 ELISA Kit;Human secreted apoptosis related protein 3 ELISA Kit;

  • Catalog
    E034429
  • species
    Human
  • GeneSFRP5
  • Standard CurveHuman SFRP5 ELISA Kit
  • Other Species Mouse Sfrp5 ELISA KitBovine SFRP5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SFRP-5. No significant cross-reactivity or interference between SFRP-5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SFRP-5 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman SFRP5 ELISA Kit allows for the in vitro quantitative determination of SFRP5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the Assay: SFRP-5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SFRP-5. Standards or samples are then added to the microtiter plate wells and SFRP-5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SFRP-5 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SFRP-5 are added to each well to "sandwich" the SFRP-5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SFRP-5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SFRP-5 concentration in each sample is interpolated from this standard curve.
  • Human Secreted frizzled-related protein 5 Protein information
  • Uniprot ID SFRP5_HUMAN
  • Uniprot AC Q5T4F7; O14780; Q86TH7;
  • UniGene Hs.279565;
  • GeneID 6425
  • KEGG hsa:6425;
  • Human Secreted frizzled-related protein 5 Protein SEQUENCE
  • SEQUENCE 317 AA; 35563 MW; 4BF060F1161751F9 CRC64;

    MRAAAAGGGV RTAALALLLG ALHWAPARCE EYDYYGWQAE PLHGRSYSKP

    PQCLDIPADL PLCHTVGYKR MRLPNLLEHE SLAEVKQQAS SWLPLLAKRC

    HSDTQVFLCS LFAPVCLDRP IYPCRSLCEA VRAGCAPLME AYGFPWPEML

    HCHKFPLDND LCIAVQFGHL PATAPPVTKI CAQCEMEHSA DGLMEQMCSS

    DFVVKMRIKE IKIENGDRKL IGAQKKKKLL KPGPLKRKDT KRLVLHMKNG

    AGCPCPQLDS LAGSFLVMGR KVDGQLLLMA VYRWDKKNKE MKFAVKFMFS

    YPCSLYYPFF YGAAEPH

  • UCSC uc001kor.5; human.;



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