Human SFRP2 ELISA Kit

Alternative name
Human FRP-2 ELISA Kit;Human sFRP-2 ELISA Kit;Human Secreted apoptosis-related protein 1 ELISA Kit;Human SARP-1 ELISA Kit;Human FRP2 ELISA Kit;Human SARP1 ELISA Kit;Human SDF-5 ELISA Kit;Human secreted frizzled related protein 2 ELISA Kit;Human secreted frizzled-related protein 2 ELISA Kit;Human secreted apoptosis related protein 1 ELISA Kit;Human testicular tissue protein Li 170 ELISA Kit;
Catalog
E034425
species
Human
GeneSFRP2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 2 (SFRP2). No significant cross-reactivity or interference between Secreted Frizzled Related Protein 2 (SFRP2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.53ng/mL.
Intended UseHuman SFRP2 ELISA Kit allows for the in vitro quantitative determination of SFRP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 2 (SFRP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 2 (SFRP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 2 (SFRP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Frizzled Related Protein 2 (SFRP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Secreted frizzled-related protein 2 Protein information
Uniprot ID SFRP2_HUMAN
Uniprot AC Q96HF1; B3KQR2; O14778; Q9HAP5;
UniGene Hs.481022;
GeneID 6423
KEGG hsa:6423;
Human Secreted frizzled-related protein 2 Protein SEQUENCE
SEQUENCE 295 AA; 33490 MW; 97597971541BFBC4 CRC64;
MLQGPGSLLL LFLASHCCLG SARGLFLFGQ PDFSYKRSNC KPIPANLQLC
HGIEYQNMRL PNLLGHETMK EVLEQAGAWI PLVMKQCHPD TKKFLCSLFA
PVCLDDLDET IQPCHSLCVQ VKDRCAPVMS AFGFPWPDML ECDRFPQDND
LCIPLASSDH LLPATEEAPK VCEACKNKND DDNDIMETLC KNDFALKIKV
KEITYINRDT KIILETKSKT IYKLNGVSER DLKKSVLWLK DSLQCTCEEM
NDINAPYLVM GQKQGGELVI TSVKRWQKGQ REFKRISRSI RKLQC
UCSC uc003inv.2; human.;

Human SFRP2 ELISA Kit

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