Human APAF1 ELISA Kit

Alternative name
Human APAF-1 ELISA Kit;Human KIAA0413 ELISA Kit;Human CED4 ELISA Kit;Human apoptotic peptidase activating factor 1 ELISA Kit;Human apoptotic protease-activating factor 1 ELISA Kit;
Catalog
E003435
species
Human
GeneAPAF1
Standard Curve
Other Species Mouse Apaf1 ELISA Kit Canine APAF1 ELISA Kit Rat Apaf1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human Apaf-1. No significant cross-reactivity or interference between human Apaf-1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human Apaf-1 is typically less than 11.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
Intended UseHuman APAF1 ELISA Kit allows for the in vitro quantitative determination of APAF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Apaf-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Apaf-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Apaf-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Apaf-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Apoptotic protease-activating factor 1 Protein information
Uniprot ID APAF_HUMAN
Uniprot AC Q9UJ65; Q9UJ66; Q9UJ67; Q9UNC9;
UniGene Hs.552567;
GeneID 317
KEGG hsa:317;
Human Apoptotic protease-activating factor 1 Protein SEQUENCE
SEQUENCE 1248 AA; 141840 MW; 0750D05817AC9B3B CRC64;
MDAKARNCLL QHREALEKDI KTSYIMDHMI SDGFLTISEE EKVRNEPTQQ
QRAAMLIKMI LKKDNDSYVS FYNALLHEGY KDLAALLHDG IPVVSSSSGK
DSVSGITSYV RTVLCEGGVP QRPVVFVTRK KLVNAIQQKL SKLKGEPGWV
TIHGMAGCGK SVLAAEAVRD HSLLEGCFPG GVHWVSVGKQ DKSGLLMKLQ
NLCTRLDQDE SFSQRLPLNI EEAKDRLRIL MLRKHPRSLL ILDDVWDSWV
LKAFDSQCQI LLTTRDKSVT DSVMGPKYVV PVESSLGKEK GLEILSLFVN
MKKADLPEQA HSIIKECKGS PLVVSLIGAL LRDFPNRWEY YLKQLQNKQF
KRIRKSSSYD YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL
CILWDMETEE VEDILQEFVN KSLLFCDRNG KSFRYYLHDL QVDFLTEKNC
SQLQDLHKKI ITQFQRYHQP HTLSPDQEDC MYWYNFLAYH MASAKMHKEL
CALMFSLDWI KAKTELVGPA HLIHEFVEYR HILDEKDCAV SENFQEFLSL
NGHLLGRQPF PNIVQLGLCE PETSEVYQQA KLQAKQEVDN GMLYLEWINK
KNITNLSRLV VRPHTDAVYH ACFSEDGQRI ASCGADKTLQ VFKAETGEKL
LEIKAHEDEV LCCAFSTDDR FIATCSVDKK VKIWNSMTGE LVHTYDEHSE
QVNCCHFTNS SHHLLLATGS SDCFLKLWDL NQKECRNTMF GHTNSVNHCR
FSPDDKLLAS CSADGTLKLW DATSANERKS INVKQFFLNL EDPQEDMEVI
VKCCSWSADG ARIMVAAKNK IFLFDIHTSG LLGEIHTGHH STIQYCDFSP
QNHLAVVALS QYCVELWNTD SRSKVADCRG HLSWVHGVMF SPDGSSFLTS
SDDQTIRLWE TKKVCKNSAV MLKQEVDVVF QENEVMVLAV DHIRRLQLIN
GRTGQIDYLT EAQVSCCCLS PHLQYIAFGD ENGAIEILEL VNNRIFQSRF
QHKKTVWHIQ FTADEKTLIS SSDDAEIQVW NWQLDKCIFL RGHQETVKDF
RLLKNSRLLS WSFDGTVKVW NIITGNKEKD FVCHQGTVLS CDISHDATKF
SSTSADKTAK IWSFDLLLPL HELRGHNGCV RCSAFSVDST LLATGDDNGE
IRIWNVSNGE LLHLCAPLSE EGAATHGGWV TDLCFSPDGK MLISAGGYIK
WWNVVTGESS QTFYTNGTNL KKIHVSPDFK TYVTVDNLGI LYILQTLE
UCSC uc001tfz.4; human. [O14727-1];

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Category

Human APAF1 ELISA Kit

Human APAF-1 ELISA Kit;Human KIAA0413 ELISA Kit;Human CED4 ELISA Kit;Human apoptotic peptidase activating factor 1 ELISA Kit;Human apoptotic protease-activating factor 1 ELISA Kit;