Human APAF1 ELISA Kit

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  • Alternative name

    Human APAF-1 ELISA Kit;Human KIAA0413 ELISA Kit;Human CED4 ELISA Kit;Human apoptotic peptidase activating factor 1 ELISA Kit;Human apoptotic protease-activating factor 1 ELISA Kit;

  • Catalog
    E003435
  • species
    Human
  • GeneAPAF1
  • Standard CurveHuman APAF1 ELISA Kit
  • Other Species Mouse Apaf1 ELISA KitCanine APAF1 ELISA KitRat Apaf1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human Apaf-1. No significant cross-reactivity or interference between human Apaf-1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human Apaf-1 is typically less than 11.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
  • Intended UseHuman APAF1 ELISA Kit allows for the in vitro quantitative determination of APAF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Apaf-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Apaf-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Apaf-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Apaf-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Apoptotic protease-activating factor 1 Protein information
  • Uniprot ID APAF_HUMAN
  • Uniprot AC Q9UJ65; Q9UJ66; Q9UJ67; Q9UNC9;
  • UniGene Hs.552567;
  • GeneID 317
  • KEGG hsa:317;
  • Human Apoptotic protease-activating factor 1 Protein SEQUENCE
  • SEQUENCE 1248 AA; 141840 MW; 0750D05817AC9B3B CRC64;

    MDAKARNCLL QHREALEKDI KTSYIMDHMI SDGFLTISEE EKVRNEPTQQ

    QRAAMLIKMI LKKDNDSYVS FYNALLHEGY KDLAALLHDG IPVVSSSSGK

    DSVSGITSYV RTVLCEGGVP QRPVVFVTRK KLVNAIQQKL SKLKGEPGWV

    TIHGMAGCGK SVLAAEAVRD HSLLEGCFPG GVHWVSVGKQ DKSGLLMKLQ

    NLCTRLDQDE SFSQRLPLNI EEAKDRLRIL MLRKHPRSLL ILDDVWDSWV

    LKAFDSQCQI LLTTRDKSVT DSVMGPKYVV PVESSLGKEK GLEILSLFVN

    MKKADLPEQA HSIIKECKGS PLVVSLIGAL LRDFPNRWEY YLKQLQNKQF

    KRIRKSSSYD YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL

    CILWDMETEE VEDILQEFVN KSLLFCDRNG KSFRYYLHDL QVDFLTEKNC

    SQLQDLHKKI ITQFQRYHQP HTLSPDQEDC MYWYNFLAYH MASAKMHKEL

    CALMFSLDWI KAKTELVGPA HLIHEFVEYR HILDEKDCAV SENFQEFLSL

    NGHLLGRQPF PNIVQLGLCE PETSEVYQQA KLQAKQEVDN GMLYLEWINK

    KNITNLSRLV VRPHTDAVYH ACFSEDGQRI ASCGADKTLQ VFKAETGEKL

    LEIKAHEDEV LCCAFSTDDR FIATCSVDKK VKIWNSMTGE LVHTYDEHSE

    QVNCCHFTNS SHHLLLATGS SDCFLKLWDL NQKECRNTMF GHTNSVNHCR

    FSPDDKLLAS CSADGTLKLW DATSANERKS INVKQFFLNL EDPQEDMEVI

    VKCCSWSADG ARIMVAAKNK IFLFDIHTSG LLGEIHTGHH STIQYCDFSP

    QNHLAVVALS QYCVELWNTD SRSKVADCRG HLSWVHGVMF SPDGSSFLTS

    SDDQTIRLWE TKKVCKNSAV MLKQEVDVVF QENEVMVLAV DHIRRLQLIN

    GRTGQIDYLT EAQVSCCCLS PHLQYIAFGD ENGAIEILEL VNNRIFQSRF

    QHKKTVWHIQ FTADEKTLIS SSDDAEIQVW NWQLDKCIFL RGHQETVKDF

    RLLKNSRLLS WSFDGTVKVW NIITGNKEKD FVCHQGTVLS CDISHDATKF

    SSTSADKTAK IWSFDLLLPL HELRGHNGCV RCSAFSVDST LLATGDDNGE

    IRIWNVSNGE LLHLCAPLSE EGAATHGGWV TDLCFSPDGK MLISAGGYIK

    WWNVVTGESS QTFYTNGTNL KKIHVSPDFK TYVTVDNLGI LYILQTLE

  • UCSC uc001tfz.4; human. [O14727-1];




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