Human Scl-70 ELISA Kit

Contact Us
  • Catalog
    E034332
  • species
    Human
  • GeneScl-70
  • Other Species Human SCL 70 ELISA KitMouse Scl-70 ELISA Kit
  • SpecificityThe microplate is coated with Scl-70 highly purified by affinity chromatography. The Anti-Scl-70 test kit is specific only for autoantibodies directed to Scl-70. No crossreactivities to the other ENA-antigens have been observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe lower detection limit for Anti-Scl-70 has been determined at 1.0 U/ml.
  • Intended UseHuman Scl-70 ELISA Kit allows for the in vitro quantitative determination of Scl-70 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyAutoimmune; Immunology; Diagnostic Assays; Assay Categories
  • Product Description
    specifical
    Principle of the assay: Highly purified Scl-70 is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.



Inquiry About Human Scl-70 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!