SpecificityThe microplate is coated with Scl-70 highly purified by affinity chromatography. The Anti-Scl-70 test kit is specific only for autoantibodies directed to Scl-70. No crossreactivities to the other ENA-antigens have been observed.
SensitivityThe lower detection limit for Anti-Scl-70 has been determined at 1.0 U/ml.
Intended UseHuman Scl-70 ELISA Kit allows for the in vitro quantitative determination of Scl-70 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: Highly purified Scl-70 is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.