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Human AIFM1 ELISA Kit

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  • Alternative name

    Human Programmed cell death protein 8 ELISA Kit;Human AIF ELISA Kit;Human PDCD8 ELISA Kit;Human CMT2D ELISA Kit;Human CMTX4 ELISA Kit;Human COWCK ELISA Kit;Human COXPD6 ELISA Kit;Human DFNX5 ELISA Kit;Human NADMR ELISA Kit;Human NAMSD ELISA Kit;Human apoptosis inducing factor mitochondria associated 1 ELISA Kit;Human apoptosis-inducing factor 1, mitochondrial ELISA Kit;Human apoptosis-inducing factor, mitochondrion-associated, 1 ELISA Kit;Human programmed cell death 8 (apoptosis-inducing factor) ELISA Kit;Human striatal apoptosis-inducing factor ELISA Kit;Human testicular secretory protein Li 4 ELISA Kit;
  • Catalog
    E003431
  • species
    Human
  • GeneAIFM1
  • Other Species Mouse Aifm1 ELISA KitRat Aifm1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human AIFM1. No significant cross-reactivity or interference between Human AIFM1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity5.8 pg/ml
  • Intended UseHuman AIFM1 ELISA Kit allows for the in vitro quantitative determination of AIFM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AIF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for AIF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain AIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AIF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Apoptosis-inducing factor 1, mitochondrial Protein information
  • Uniprot ID AIFM1_HUMAN
  • Uniprot AC Q5JUZ7; Q6I9X6; Q9Y3I3; Q9Y3I4;
  • UniGene Hs.424932;
  • GeneID 9131
  • KEGG hsa:9131;
  • Human Apoptosis-inducing factor 1, mitochondrial Protein SEQUENCE

  • SEQUENCE 613 AA; 66901 MW; A156762BC64E6340 CRC64;

    MFRCGGLAAG ALKQKLVPLV RTVCVRSPRQ RNRLPGNLFQ RWHVPLELQM

    TRQMASSGAS GGKIDNSVLV LIVGLSTVGA GAYAYKTMKE DEKRYNERIS

    GLGLTPEQKQ KKAALSASEG EEVPQDKAPS HVPFLLIGGG TAAFAAARSI

    RARDPGARVL IVSEDPELPY MRPPLSKELW FSDDPNVTKT LRFKQWNGKE

    RSIYFQPPSF YVSAQDLPHI ENGGVAVLTG KKVVQLDVRD NMVKLNDGSQ

    ITYEKCLIAT GGTPRSLSAI DRAGAEVKSR TTLFRKIGDF RSLEKISREV

    KSITIIGGGF LGSELACALG RKARALGTEV IQLFPEKGNM GKILPEYLSN

    WTMEKVRREG VKVMPNAIVQ SVGVSSGKLL IKLKDGRKVE TDHIVAAVGL

    EPNVELAKTG GLEIDSDFGG FRVNAELQAR SNIWVAGDAA CFYDIKLGRR

    RVEHHDHAVV SGRLAGENMT GAAKPYWHQS MFWSDLGPDV GYEAIGLVDS

    SLPTVGVFAK ATAQDNPKSA TEQSGTGIRS ESETESEASE ITIPPSTPAV

    PQAPVQGEDY GKGVIFYLRD KVVVGIVLWN IFNRMPIARK IIKDGEQHED

    LNEVAKLFNI HED

  • UCSC uc004evg.4; human. [O95831-1];


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