Human sCD14 ELISA Kit

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  • Catalog
    E034285
  • species
    Human
  • GenesCD14
  • Other Species Mouse Scd14 ELISA Kit
  • SpecificityThis kit recognizes natural and recombinant Human sCD14. No significant cross-reactivity or interference between Human sCD14 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.188ng/mL
  • Intended UseHuman sCD14 ELISA Kit allows for the in vitro quantitative determination of sCD14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human sCD14 concentrations in serum, plasma and other biological fluids. Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to sCD14. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for sCD14 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain sCD14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of sCD14. You can calculate the concentration of sCD14 in the samples by comparing the OD of the samples to the standard curve.


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