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Alternative name
Protein S100-A8 ELISA KIT; Calgranulin-A ELISA KIT; Calprotectin L1L subunit ELISA KIT; Cystic fibrosis antigen ELISA KIT; CFAG ELISA KIT; Leukocyte L1 complex light chain ELISA KIT; Migration inhibitory factor-related protein 8 ELISA KIT; MRP-8 ELISA KIT; p8 ELISA KIT; S100 calcium-binding protein A8 ELISA KIT; Urinary stone protein band ACleaved into the following chain:Protein S100-A8, N-terminally processedS100A8 ELISA KIT; CAGA ELISA KIT; CFAG ELISA KIT; MRP8 ELISA KIT; CFAG ELISA KIT; MRP-8 ELISA KIT; p8 ELISA KIT
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Catalog
E034128
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species
Human
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GeneS100A8/A9
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human S100A8/A9. No significant cross-reactivity or interference between human S100A8/A9 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.39 ng/ml.
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Intended UseHuman S100A8/A9 ELISA Kit allows for the in vitro quantitative determination of S100A8/A9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100A8/A9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A8/A9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A8/A9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A8/A9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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