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Human S100B ELISA Kit

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  • Alternative name

    Human S-100 protein beta chain ELISA Kit;Human S-100 protein subunit beta ELISA Kit;Human S100 calcium-binding protein B ELISA Kit;Human NEF ELISA Kit;Human S100 ELISA Kit;Human S100-B ELISA Kit;Human S100beta ELISA Kit;Human S100 calcium binding protein B ELISA Kit;Human protein S100-B ELISA Kit;Human S-100 calcium-binding protein, beta chain ELISA Kit;Human S100 calcium-binding protein, beta (neural) ELISA Kit;
  • Catalog
    E034117
  • species
    Human
  • GeneS100B
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein B (S100B). No significant cross-reactivity or interference between S100 Calcium Binding Protein B (S100B) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.064ng/mL.
  • Intended UseHuman S100B ELISA Kit allows for the in vitro quantitative determination of S100B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein B (S100B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein B (S100B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein B (S100B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein B (S100B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein S100-B Protein information
  • Uniprot ID S100B_HUMAN
  • Uniprot AC P04271; D3DSN6;
  • UniGene Hs.422181;
  • GeneID 6285
  • KEGG hsa:6285;
  • Human Protein S100-B Protein SEQUENCE

  • SEQUENCE 92 AA; 10713 MW; 43815AC212A3AD6B CRC64;

    MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE

    QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE

  • UCSC uc002zju.2; human.;



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