Human S100A5 ELISA Kit

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  • Alternative name

    Human Protein S-100D ELISA Kit;Human S100 calcium-binding protein A5 ELISA Kit;Human S100D ELISA Kit;Human S100 calcium binding protein A5 ELISA Kit;Human protein S100-A5 ELISA Kit;

  • Catalog
    E034097
  • species
    Human
  • GeneS100A5
  • Standard CurveHuman S100A5 ELISA Kit
  • Other Species Mouse S100a5 ELISA KitRat S100a5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A5 (S100A5). No significant cross-reactivity or interference between S100 Calcium Binding Protein A5 (S100A5) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.042ng/mL.
  • Intended UseHuman S100A5 ELISA Kit allows for the in vitro quantitative determination of S100A5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A5 (S100A5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A5 (S100A5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A5 (S100A5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A5 (S100A5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein S100-A5 Protein information
  • Uniprot ID S10A5_HUMAN
  • Uniprot AC P33763; Q52LE7; Q5RHS3;
  • UniGene Hs.2960;
  • GeneID 6276
  • KEGG hsa:6276;
  • Human Protein S100-A5 Protein SEQUENCE
  • SEQUENCE 92 AA; 10744 MW; 49C2B1DAEC481561 CRC64;

    METPLEKALT TMVTTFHKYS GREGSKLTLS RKELKELIKK ELCLGEMKES

    SIDDLMKSLD KNSDQEIDFK EYSVFLTMLC MAYNDFFLED NK

  • UCSC uc001fbx.3; human. [P33763-1];


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