Human S100A12 ELISA Kit

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  • Alternative name

    Human CGRP ELISA Kit;Human calcium-binding protein in amniotic fluid 1 ELISA Kit;Human CAAF1 ELISA Kit;Human Calgranulin-C ELISA Kit;Human CAGC ELISA Kit;Human extracellular newly identified RAGE-binding protein ELISA Kit;Human EN-RAGE ELISA Kit;Human migration inhibitory factor-related protein 6 ELISA Kit;Human MRP-6 ELISA Kit;Human p6 ELISA Kit;Human neutrophil S100 protein ELISA Kit;Human S100 calcium-binding protein A12 ELISA Kit;Human ENRAGE ELISA Kit;Human MRP6 ELISA Kit;Human S100 calcium binding protein A12 ELISA Kit;Human protein S100-A12 ELISA Kit;Human calcitermin ELISA Kit;Human calgranulin C ELISA Kit;

  • Catalog
    E034075
  • species
    Human
  • GeneS100A12
  • Standard CurveHuman S100A12 ELISA Kit
  • Other Species Mouse S100A12 ELISA KitBovine S100A12 ELISA KitPorcine S100A12 ELISA KitRabbit S100A12 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman S100A12 ELISA Kit allows for the in vitro quantitative determination of S100A12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This S100A12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human S100A12. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: S100A12 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for S100A12. Standards or samples are then added to the microtiter plate wells and S100A12 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of S100A12 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for S100A12 are added to each well to "sandwich" the S100A12 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain S100A12 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A12 concentration in each sample is interpolated from this standard curve.
  • Human Calcitermin Protein information
  • Uniprot ID S10AC_HUMAN
  • Uniprot AC P80511; P83219; Q5SY66; Q7M4R1;
  • UniGene Hs.19413;
  • GeneID 6283
  • KEGG hsa:6283;
  • Human Calcitermin Protein SEQUENCE
  • SEQUENCE 92 AA; 10575 MW; 52AF75A31BDC222A CRC64;

    MTKLEEHLEG IVNIFHQYSV RKGHFDTLSK GELKQLLTKE LANTIKNIKD

    KAVIDEIFQG LDANQDEQVD FQEFISLVAI ALKAAHYHTH KE

  • UCSC uc001fbr.2; human.;


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