SpecificityThis assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A11 (S100A11).
No significant cross-reactivity or interference between S100 Calcium Binding Protein A11 (S100A11) and analogues was observed.
Intended UseHuman S100A11 ELISA Kit allows for the in vitro quantitative determination of S100A11 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A11 (S100A11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A11 (S100A11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A11 (S100A11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A11 (S100A11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Protein S100-A11, N-terminally processed Protein information
Human Protein S100-A11, N-terminally processed Protein SEQUENCE