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Human API5 ELISA Kit

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  • Alternative name

    Human API-5 ELISA Kit;Human antiapoptosis clone 11 protein ELISA Kit;Human AAC-11 ELISA Kit;Human cell migration-inducing gene 8 protein ELISA Kit;Human Fibroblast growth factor 2-interacting factor ELISA Kit;Human FIF ELISA Kit;Human Protein XAGL ELISA Kit;Human AAC11 ELISA Kit;Human apoptosis inhibitor 5 ELISA Kit;Human fibroblast growth factor 2-interacting factor 2 ELISA Kit;Human migration-inducing protein MIG8 ELISA Kit;
  • Catalog
    E003407
  • species
    Human
  • GeneAPI5
  • Standard CurveHuman API5 ELISA Kit
  • Other Species Mouse Api5 ELISA KitChicken API5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Apoptosis Inhibitor 5 (API5). No significant cross-reactivity or interference between Apoptosis Inhibitor 5 (API5) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.122ng/mL.
  • Intended UseHuman API5 ELISA Kit allows for the in vitro quantitative determination of API5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptosis Inhibitor 5 (API5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apoptosis Inhibitor 5 (API5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apoptosis Inhibitor 5 (API5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apoptosis Inhibitor 5 (API5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Apoptosis inhibitor 5 Protein information
  • Uniprot ID API5_HUMAN
  • Uniprot AC Q9BZZ5; B4DGR0; B4DRJ2; B4E283; D3DR21; O15441; Q9Y4J7;
  • UniGene Hs.435771;
  • GeneID 8539
  • KEGG hsa:8539;
  • Human Apoptosis inhibitor 5 Protein SEQUENCE

  • SEQUENCE 524 AA; 59005 MW; A2EA6BE4D8318BB9 CRC64;

    MPTVEELYRN YGILADATEQ VGQHKDAYQV ILDGVKGGTK EKRLAAQFIP

    KFFKHFPELA DSAINAQLDL CEDEDVSIRR QAIKELPQFA TGENLPRVAD

    ILTQLLQTDD SAEFNLVNNA LLSIFKMDAK GTLGGLFSQI LQGEDIVRER

    AIKFLSTKLK TLPDEVLTKE VEELILTESK KVLEDVTGEE FVLFMKILSG

    LKSLQTVSGR QQLVELVAEQ ADLEQTFNPS DPDCVDRLLQ CTRQAVPLFS

    KNVHSTRFVT YFCEQVLPNL GTLTTPVEGL DIQLEVLKLL AEMSSFCGDM

    EKLETNLRKL FDKLLEYMPL PPEEAENGEN AGNEEPKLQF SYVECLLYSF

    HQLGRKLPDF LTAKLNAEKL KDFKIRLQYF ARGLQVYIRQ LRLALQGKTG

    EALKTEENKI KVVALKITNN INVLIKDLFH IPPSYKSTVT LSWKPVQKVE

    IGQKRASEDT TSGSPPKKSS AGPKRDARQI YNPPSGKYSS NLGNFNYEQR

    GAFRGSRGGR GWGTRGNRSR GRLY

  • UCSC uc010rfg.2; human.;


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