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Alternative name
Human S-100 protein alpha chain ELISA Kit;Human S-100 protein subunit alpha ELISA Kit;Human S100 calcium-binding protein A1 ELISA Kit;Human S100A ELISA Kit;Human S100 ELISA Kit;Human S100-alpha ELISA Kit;Human S100 calcium binding protein A1 ELISA Kit;Human protein S100-A1 ELISA Kit;Human S100 alpha ELISA Kit;Human S100 protein, alpha polypeptide ELISA Kit;
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Catalog
E034069
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species
Human
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GeneS100A1
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Other Species
Human S100A1/S100 ELISA KitMouse S100a1 ELISA KitBovine S100A1 ELISA KitRat S100a1 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Human S100A1. No significant cross-reactivity or interference between Human S100A1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.04 ng/ml
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Intended UseHuman S100A1 ELISA Kit allows for the in vitro quantitative determination of S100A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to S-100. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for S-100 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain S-100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of S-100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Human Protein S100-A1 Protein information
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Uniprot ID
S10A1_HUMAN
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Uniprot AC
P23297; B2R5D9; Q5T7Y3;
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UniGene
Hs.515715;
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GeneID
6271
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KEGG
hsa:6271;
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Human Protein S100-A1 Protein SEQUENCE
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SEQUENCE 94 AA; 10546 MW; AD5E53AF326B25D2 CRC64;
MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKELLQT ELSGFLDAQK
DVDAVDKVMK
ELDENGDGEV DFQEYVVLVA ALTVACNNFF WENS
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UCSC
uc001fck.1; human.;
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