Human rT3 ELISA Kit

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  • Alternative name

    Retrotransposon protein ELISA KIT; Retrotransposon proteinRT3 ELISA KIT;

  • Catalog
    E033987
  • species
    Human
  • GenerT3
  • Other Species Mouse rT3 ELISA Kit
  • SpecificityThis kit recognizes natural and recombinant Human rT3. No significant cross-reactivity or interference between Human rT3 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity56.25pg/mL
  • Intended UseHuman rT3 ELISA Kit allows for the in vitro quantitative determination of rT3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human rT3 concentrations in serum, plasma and other biological fluids. Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with rT3. During the reaction, rT3 in the sample or standard competes with a fixed amount of rT3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to rT3. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve.


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