Human ROBO1 ELISA Kit

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  • Alternative name

    Human deleted in U twenty twenty ELISA Kit;Human H-Robo-1 ELISA Kit;Human DUTT1 ELISA Kit;Human SAX3 ELISA Kit;Human roundabout guidance receptor 1 ELISA Kit;Human roundabout homolog 1 ELISA Kit;Human roundabout, axon guidance receptor, homolog 1 ELISA Kit;

  • Catalog
    E033971
  • species
    Human
  • GeneROBO1
  • Standard CurveHuman ROBO1 ELISA Kit
  • Other Species Mouse Robo1 ELISA KitRat Robo1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1). No significant cross-reactivity or interference between Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.118ng/mL.
  • Intended UseHuman ROBO1 ELISA Kit allows for the in vitro quantitative determination of ROBO1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Roundabout homolog 1 Protein information
  • Uniprot ID ROBO1_HUMAN
  • Uniprot AC Q9Y6N7; B2RXI1; D3DU36; E9PD49; Q1RMC7; Q7Z300; Q9BUS7;
  • UniGene Hs.744218;
  • GeneID 6091
  • KEGG hsa:6091;
  • Human Roundabout homolog 1 Protein SEQUENCE
  • SEQUENCE 1651 AA; 180930 MW; 9D98CD7CAB73074D CRC64;

    MKWKHVPFLV MISLLSLSPN HLFLAQLIPD PEDVERGNDH GTPIPTSDND

    DNSLGYTGSR LRQEDFPPRI VEHPSDLIVS KGEPATLNCK AEGRPTPTIE

    WYKGGERVET DKDDPRSHRM LLPSGSLFFL RIVHGRKSRP DEGVYVCVAR

    NYLGEAVSHN ASLEVAILRD DFRQNPSDVM VAVGEPAVME CQPPRGHPEP

    TISWKKDGSP LDDKDERITI RGGKLMITYT RKSDAGKYVC VGTNMVGERE

    SEVAELTVLE RPSFVKRPSN LAVTVDDSAE FKCEARGDPV PTVRWRKDDG

    ELPKSRYEIR DDHTLKIRKV TAGDMGSYTC VAENMVGKAE ASATLTVQEP

    PHFVVKPRDQ VVALGRTVTF QCEATGNPQP AIFWRREGSQ NLLFSYQPPQ

    SSSRFSVSQT GDLTITNVQR SDVGYYICQT LNVAGSIITK AYLEVTDVIA

    DRPPPVIRQG PVNQTVAVDG TFVLSCVATG SPVPTILWRK DGVLVSTQDS

    RIKQLENGVL QIRYAKLGDT GRYTCIASTP SGEATWSAYI EVQEFGVPVQ

    PPRPTDPNLI PSAPSKPEVT DVSRNTVTLS WQPNLNSGAT PTSYIIEAFS

    HASGSSWQTV AENVKTETSA IKGLKPNAIY LFLVRAANAY GISDPSQISD

    PVKTQDVLPT SQGVDHKQVQ RELGNAVLHL HNPTVLSSSS IEVHWTVDQQ

    SQYIQGYKIL YRPSGANHGE SDWLVFEVRT PAKNSVVIPD LRKGVNYEIK

    ARPFFNEFQG ADSEIKFAKT LEEAPSAPPQ GVTVSKNDGN GTAILVSWQP

    PPEDTQNGMV QEYKVWCLGN ETRYHINKTV DGSTFSVVIP FLVPGIRYSV

    EVAASTGAGS GVKSEPQFIQ LDAHGNPVSP EDQVSLAQQI SDVVKQPAFI

    AGIGAACWII LMVFSIWLYR HRKKRNGLTS TYAGIRKVPS FTFTPTVTYQ

    RGGEAVSSGG RPGLLNISEP AAQPWLADTW PNTGNNHNDC SISCCTAGNG

    NSDSNLTTYS RPADCIANYN NQLDNKQTNL MLPESTVYGD VDLSNKINEM

    KTFNSPNLKD GRFVNPSGQP TPYATTQLIQ SNLSNNMNNG SGDSGEKHWK

    PLGQQKQEVA PVQYNIVEQN KLNKDYRAND TVPPTIPYNQ SYDQNTGGSY

    NSSDRGSSTS GSQGHKKGAR TPKVPKQGGM NWADLLPPPP AHPPPHSNSE

    EYNISVDESY DQEMPCPVPP ARMYLQQDEL EEEEDERGPT PPVRGAASSP

    AAVSYSHQST ATLTPSPQEE LQPMLQDCPE ETGHMQHQPD RRRQPVSPPP

    PPRPISPPHT YGYISGPLVS DMDTDAPEEE EDEADMEVAK MQTRRLLLRG

    LEQTPASSVG DLESSVTGSM INGWGSASEE DNISSGRSSV SSSDGSFFTD

    ADFAQAVAAA AEYAGLKVAR RQMQDAAGRR HFHASQCPRP TSPVSTDSNM

    SAAVMQKTRP AKKLKHQPGH LRRETYTDDL PPPPVPPPAI KSPTAQSKTQ

    LEVRPVVVPK LPSMDARTDR SSDRKGSSYK GREVLDGRQV VDMRTNPGDP

    REAQEQQNDG KGRGNKAAKR DLPPAKTHLI QEDILPYCRP TFPTSNNPRD

    PSSSSSMSSR GSGSRQREQA NVGRRNIAEM QVLGGYERGE DNNEELEETE

    S

  • UCSC uc003dqc.3; human. [Q9Y6N7-1];


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